Transcriptome database KK-Periome for periodontal ligament development: Expression profiles of the extracellular matrix genes

Eisaku Nishida, Takashi Sasaki, Sabine Kazuko Ishikawa, Kazutaka Kosaka, Makoto Aino, Toshihide Noguchi, Toshio Teranaka, Nobuyoshi Shimizu, Masahiro Saito

研究成果: Article

32 引用 (Scopus)

抄録

Specialized connective tissues such as tendon/ligament develop through a series of events that require temporal and spatial expression of numerous genes in mesenchymal progenitors. However, the genes required for tendon/ligament development have not been identified yet. To solve this problem, we made a cDNA library from periodontal ligament and sequenced 11,520 cDNA clones, as a model for investigating tendon/ligament development. The resulting sequence data was assembled to 617 expressed sequence tag (EST) clusters, and an EST database for human periodontal ligament (PDL) was constructed (designated as the KK-Periome database). In the KK-Periome database, the top 13 EST clusters were related to extracellular matrix (ECM) genes. The temporal and spatial expression patterns of these genes during mouse PDL development were examined by in situ hybridization. Among these genes, F-spondin was expressed specifically in dental follicle (DF) cells during tooth germ development, whereas tenascin-N was strongly expressed in the terminally differentiated PDL. This characteristic expression profile was confirmed by in vivo differentiation assay of human PDL (hPDL) cells in the mouse transplant. Thus, the KK-Periome database was proven to be a useful resource for PDL-derived ESTs (transcriptome), and in fact, initial evidence indicated that F-spondin and tenascin-N might serve as markers for DF and PDL, respectively.

元の言語English
ページ(範囲)70-79
ページ数10
ジャーナルGene
404
発行部数1-2
DOI
出版物ステータスPublished - 2007 12 1

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Periodontal Ligament
Transcriptome
Extracellular Matrix
Databases
Expressed Sequence Tags
Genes
Dental Sac
Ligaments
Tendons
Tenascin
Tooth Germ
Gene Expression
Gene Library
Connective Tissue
In Situ Hybridization
Complementary DNA
Clone Cells
Transplants

ASJC Scopus subject areas

  • Genetics

これを引用

Transcriptome database KK-Periome for periodontal ligament development : Expression profiles of the extracellular matrix genes. / Nishida, Eisaku; Sasaki, Takashi; Ishikawa, Sabine Kazuko; Kosaka, Kazutaka; Aino, Makoto; Noguchi, Toshihide; Teranaka, Toshio; Shimizu, Nobuyoshi; Saito, Masahiro.

:: Gene, 巻 404, 番号 1-2, 01.12.2007, p. 70-79.

研究成果: Article

Nishida, E, Sasaki, T, Ishikawa, SK, Kosaka, K, Aino, M, Noguchi, T, Teranaka, T, Shimizu, N & Saito, M 2007, 'Transcriptome database KK-Periome for periodontal ligament development: Expression profiles of the extracellular matrix genes', Gene, 巻. 404, 番号 1-2, pp. 70-79. https://doi.org/10.1016/j.gene.2007.09.009
Nishida, Eisaku ; Sasaki, Takashi ; Ishikawa, Sabine Kazuko ; Kosaka, Kazutaka ; Aino, Makoto ; Noguchi, Toshihide ; Teranaka, Toshio ; Shimizu, Nobuyoshi ; Saito, Masahiro. / Transcriptome database KK-Periome for periodontal ligament development : Expression profiles of the extracellular matrix genes. :: Gene. 2007 ; 巻 404, 番号 1-2. pp. 70-79.
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abstract = "Specialized connective tissues such as tendon/ligament develop through a series of events that require temporal and spatial expression of numerous genes in mesenchymal progenitors. However, the genes required for tendon/ligament development have not been identified yet. To solve this problem, we made a cDNA library from periodontal ligament and sequenced 11,520 cDNA clones, as a model for investigating tendon/ligament development. The resulting sequence data was assembled to 617 expressed sequence tag (EST) clusters, and an EST database for human periodontal ligament (PDL) was constructed (designated as the KK-Periome database). In the KK-Periome database, the top 13 EST clusters were related to extracellular matrix (ECM) genes. The temporal and spatial expression patterns of these genes during mouse PDL development were examined by in situ hybridization. Among these genes, F-spondin was expressed specifically in dental follicle (DF) cells during tooth germ development, whereas tenascin-N was strongly expressed in the terminally differentiated PDL. This characteristic expression profile was confirmed by in vivo differentiation assay of human PDL (hPDL) cells in the mouse transplant. Thus, the KK-Periome database was proven to be a useful resource for PDL-derived ESTs (transcriptome), and in fact, initial evidence indicated that F-spondin and tenascin-N might serve as markers for DF and PDL, respectively.",
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AU - Kosaka, Kazutaka

AU - Aino, Makoto

AU - Noguchi, Toshihide

AU - Teranaka, Toshio

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AB - Specialized connective tissues such as tendon/ligament develop through a series of events that require temporal and spatial expression of numerous genes in mesenchymal progenitors. However, the genes required for tendon/ligament development have not been identified yet. To solve this problem, we made a cDNA library from periodontal ligament and sequenced 11,520 cDNA clones, as a model for investigating tendon/ligament development. The resulting sequence data was assembled to 617 expressed sequence tag (EST) clusters, and an EST database for human periodontal ligament (PDL) was constructed (designated as the KK-Periome database). In the KK-Periome database, the top 13 EST clusters were related to extracellular matrix (ECM) genes. The temporal and spatial expression patterns of these genes during mouse PDL development were examined by in situ hybridization. Among these genes, F-spondin was expressed specifically in dental follicle (DF) cells during tooth germ development, whereas tenascin-N was strongly expressed in the terminally differentiated PDL. This characteristic expression profile was confirmed by in vivo differentiation assay of human PDL (hPDL) cells in the mouse transplant. Thus, the KK-Periome database was proven to be a useful resource for PDL-derived ESTs (transcriptome), and in fact, initial evidence indicated that F-spondin and tenascin-N might serve as markers for DF and PDL, respectively.

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