Transcriptomic analysis reveals differences in the regulation of amino acid metabolism in asexual and sexual planarians

Kiyono Sekii, Shunta Yorimoto, Hikaru Okamoto, Nanna Nagao, Takanobu Maezawa, Yasuhisa Matsui, Katsushi Yamaguchi, Ryohei Furukawa, Shuji Shigenobu, Kazuya Kobayashi

研究成果: Article

2 引用 (Scopus)

抄録

Many flatworms can alternate between asexual and sexual reproduction. This is a powerful reproductive strategy enabling them to benefit from the features of the two reproductive modes, namely, rapid multiplication and genetic shuffling. The two reproductive modes are enabled by the presence of pluripotent adult stem cells (neoblasts), by generating any type of tissue in the asexual mode, and producing and maintaining germ cells in the sexual mode. In the current study, RNA sequencing (RNA-seq) was used to compare the transcriptomes of two phenotypes of the planarian Dugesia ryukyuensis: an asexual OH strain and an experimentally sexualized OH strain. Pathway enrichment analysis revealed striking differences in amino acid metabolism in the two worm types. Further, the analysis identified serotonin as a new bioactive substance that induced the planarian ovary de novo in a postembryonic manner. These findings suggest that different metabolic states and physiological conditions evoked by sex-inducing substances likely modulate stem cell behavior, depending on their different function in the asexual and sexual reproductive modes. The combination of RNA-seq and a feeding assay in D. ryukyuensis is a powerful tool for studying the alternation of reproductive modes, disentangling the relationship between gene expression and chemical signaling molecules.

元の言語English
記事番号6132
ジャーナルScientific reports
9
発行部数1
DOI
出版物ステータスPublished - 2019 12 1

Fingerprint

Planarians
RNA Sequence Analysis
Amino Acids
Asexual Reproduction
Platyhelminths
Pluripotent Stem Cells
Adult Stem Cells
Transcriptome
Germ Cells
Ovary
Serotonin
Stem Cells
Phenotype
Gene Expression

ASJC Scopus subject areas

  • General

これを引用

Transcriptomic analysis reveals differences in the regulation of amino acid metabolism in asexual and sexual planarians. / Sekii, Kiyono; Yorimoto, Shunta; Okamoto, Hikaru; Nagao, Nanna; Maezawa, Takanobu; Matsui, Yasuhisa; Yamaguchi, Katsushi; Furukawa, Ryohei; Shigenobu, Shuji; Kobayashi, Kazuya.

:: Scientific reports, 巻 9, 番号 1, 6132, 01.12.2019.

研究成果: Article

Sekii, K, Yorimoto, S, Okamoto, H, Nagao, N, Maezawa, T, Matsui, Y, Yamaguchi, K, Furukawa, R, Shigenobu, S & Kobayashi, K 2019, 'Transcriptomic analysis reveals differences in the regulation of amino acid metabolism in asexual and sexual planarians', Scientific reports, 巻. 9, 番号 1, 6132. https://doi.org/10.1038/s41598-019-42025-z
Sekii, Kiyono ; Yorimoto, Shunta ; Okamoto, Hikaru ; Nagao, Nanna ; Maezawa, Takanobu ; Matsui, Yasuhisa ; Yamaguchi, Katsushi ; Furukawa, Ryohei ; Shigenobu, Shuji ; Kobayashi, Kazuya. / Transcriptomic analysis reveals differences in the regulation of amino acid metabolism in asexual and sexual planarians. :: Scientific reports. 2019 ; 巻 9, 番号 1.
@article{259af8c86f314004ae3bad5e263b3b92,
title = "Transcriptomic analysis reveals differences in the regulation of amino acid metabolism in asexual and sexual planarians",
abstract = "Many flatworms can alternate between asexual and sexual reproduction. This is a powerful reproductive strategy enabling them to benefit from the features of the two reproductive modes, namely, rapid multiplication and genetic shuffling. The two reproductive modes are enabled by the presence of pluripotent adult stem cells (neoblasts), by generating any type of tissue in the asexual mode, and producing and maintaining germ cells in the sexual mode. In the current study, RNA sequencing (RNA-seq) was used to compare the transcriptomes of two phenotypes of the planarian Dugesia ryukyuensis: an asexual OH strain and an experimentally sexualized OH strain. Pathway enrichment analysis revealed striking differences in amino acid metabolism in the two worm types. Further, the analysis identified serotonin as a new bioactive substance that induced the planarian ovary de novo in a postembryonic manner. These findings suggest that different metabolic states and physiological conditions evoked by sex-inducing substances likely modulate stem cell behavior, depending on their different function in the asexual and sexual reproductive modes. The combination of RNA-seq and a feeding assay in D. ryukyuensis is a powerful tool for studying the alternation of reproductive modes, disentangling the relationship between gene expression and chemical signaling molecules.",
author = "Kiyono Sekii and Shunta Yorimoto and Hikaru Okamoto and Nanna Nagao and Takanobu Maezawa and Yasuhisa Matsui and Katsushi Yamaguchi and Ryohei Furukawa and Shuji Shigenobu and Kazuya Kobayashi",
year = "2019",
month = "12",
day = "1",
doi = "10.1038/s41598-019-42025-z",
language = "English",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Transcriptomic analysis reveals differences in the regulation of amino acid metabolism in asexual and sexual planarians

AU - Sekii, Kiyono

AU - Yorimoto, Shunta

AU - Okamoto, Hikaru

AU - Nagao, Nanna

AU - Maezawa, Takanobu

AU - Matsui, Yasuhisa

AU - Yamaguchi, Katsushi

AU - Furukawa, Ryohei

AU - Shigenobu, Shuji

AU - Kobayashi, Kazuya

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Many flatworms can alternate between asexual and sexual reproduction. This is a powerful reproductive strategy enabling them to benefit from the features of the two reproductive modes, namely, rapid multiplication and genetic shuffling. The two reproductive modes are enabled by the presence of pluripotent adult stem cells (neoblasts), by generating any type of tissue in the asexual mode, and producing and maintaining germ cells in the sexual mode. In the current study, RNA sequencing (RNA-seq) was used to compare the transcriptomes of two phenotypes of the planarian Dugesia ryukyuensis: an asexual OH strain and an experimentally sexualized OH strain. Pathway enrichment analysis revealed striking differences in amino acid metabolism in the two worm types. Further, the analysis identified serotonin as a new bioactive substance that induced the planarian ovary de novo in a postembryonic manner. These findings suggest that different metabolic states and physiological conditions evoked by sex-inducing substances likely modulate stem cell behavior, depending on their different function in the asexual and sexual reproductive modes. The combination of RNA-seq and a feeding assay in D. ryukyuensis is a powerful tool for studying the alternation of reproductive modes, disentangling the relationship between gene expression and chemical signaling molecules.

AB - Many flatworms can alternate between asexual and sexual reproduction. This is a powerful reproductive strategy enabling them to benefit from the features of the two reproductive modes, namely, rapid multiplication and genetic shuffling. The two reproductive modes are enabled by the presence of pluripotent adult stem cells (neoblasts), by generating any type of tissue in the asexual mode, and producing and maintaining germ cells in the sexual mode. In the current study, RNA sequencing (RNA-seq) was used to compare the transcriptomes of two phenotypes of the planarian Dugesia ryukyuensis: an asexual OH strain and an experimentally sexualized OH strain. Pathway enrichment analysis revealed striking differences in amino acid metabolism in the two worm types. Further, the analysis identified serotonin as a new bioactive substance that induced the planarian ovary de novo in a postembryonic manner. These findings suggest that different metabolic states and physiological conditions evoked by sex-inducing substances likely modulate stem cell behavior, depending on their different function in the asexual and sexual reproductive modes. The combination of RNA-seq and a feeding assay in D. ryukyuensis is a powerful tool for studying the alternation of reproductive modes, disentangling the relationship between gene expression and chemical signaling molecules.

UR - http://www.scopus.com/inward/record.url?scp=85064552805&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85064552805&partnerID=8YFLogxK

U2 - 10.1038/s41598-019-42025-z

DO - 10.1038/s41598-019-42025-z

M3 - Article

C2 - 30992461

AN - SCOPUS:85064552805

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 6132

ER -