Transfection analysis of functional roles of complexin I and II in the exocytosis of two different types of secretory vesicles

Makoto Itakura, Hidemi Misawa, Mariko Sekiguchi, Seiichi Takahashi, Masami Takahashi

研究成果: Article査読

64 被引用数 (Scopus)

抄録

Classical neurotransmitters such as γ-aminobutyric acid and glutamate are released from synaptic nerve terminals by exocytosis of synaptic vesicles. PC12 cells also have SSVs capable of storing acetylcholine (ACh). A novel method to examine the effect of transient transfection of any gene of interest on the exocytosis of SSVs was developed. The transfection of choline acetyltransferase (ChAT) into PC12 cells which have lost ACh synthesizing activity resulted in the accumulation of a substantial amount of ACh. Synthesized ACh was released in Ca2+-dependent manner. Release was thought to occur by an exocytosis of SSVs because: (1) release was abolished by treating the cells with vesamicol, a specific inhibitor of the vesicular ACh transporter (VAChT) localizing specifically in SSVs; and (2) the release was further increased by cotransfecting rat VAChT with the ChAT. By means of this method, we showed that overexpression of complexin I or II with ChAT markedly suppressed high-K+-dependent ACh release of SSVs.

本文言語English
ページ(範囲)691-696
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
265
3
DOI
出版ステータスPublished - 1999 11 30
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Transfection analysis of functional roles of complexin I and II in the exocytosis of two different types of secretory vesicles」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル