Transformation of intestinal stem cells into gastric stem cells on loss of transcription factor Cdx2

Salvatore Simmini, Monika Bialecka, Meritxell Huch, Lennart Kester, Marc Van De Wetering, Toshiro Sato, Felix Beck, Alexander Van Oudenaarden, Hans Clevers, Jacqueline Deschamps

研究成果: Article査読

60 被引用数 (Scopus)

抄録

The endodermal lining of the adult gastro-intestinal tract harbours stem cells that are responsible for the day-to-day regeneration of the epithelium. Stem cells residing in the pyloric glands of the stomach and in the small intestinal crypts differ in their differentiation programme and in the gene repertoire that they express. Both types of stem cells have been shown to grow from single cells into 3D structures (organoids) in vitro. We show that single adult Lgr5-positive stem cells, isolated from small intestinal organoids, require Cdx2 to maintain their intestinal identity and are converted cell-autonomously into pyloric stem cells in the absence of this transcription factor. Clonal descendants of Cdx2 null small intestinal stem cells enter the gastric differentiation program instead of producing intestinal derivatives. We show that the intestinal genetic programme is critically dependent on the single transcription factor encoding gene Cdx2.

本文言語English
論文番号5728
ジャーナルNature communications
5
DOI
出版ステータスPublished - 2014

ASJC Scopus subject areas

  • 化学 (全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 物理学および天文学(全般)

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