Transformation of intestinal stem cells into gastric stem cells on loss of transcription factor Cdx2

Salvatore Simmini, Monika Bialecka, Meritxell Huch, Lennart Kester, Marc Van De Wetering, Toshiro Sato, Felix Beck, Alexander Van Oudenaarden, Hans Clevers, Jacqueline Deschamps

研究成果: Article

43 引用 (Scopus)

抜粋

The endodermal lining of the adult gastro-intestinal tract harbours stem cells that are responsible for the day-to-day regeneration of the epithelium. Stem cells residing in the pyloric glands of the stomach and in the small intestinal crypts differ in their differentiation programme and in the gene repertoire that they express. Both types of stem cells have been shown to grow from single cells into 3D structures (organoids) in vitro. We show that single adult Lgr5-positive stem cells, isolated from small intestinal organoids, require Cdx2 to maintain their intestinal identity and are converted cell-autonomously into pyloric stem cells in the absence of this transcription factor. Clonal descendants of Cdx2 null small intestinal stem cells enter the gastric differentiation program instead of producing intestinal derivatives. We show that the intestinal genetic programme is critically dependent on the single transcription factor encoding gene Cdx2.

元の言語English
記事番号6728
ジャーナルNature communications
5
DOI
出版物ステータスPublished - 2014

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ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

これを引用

Simmini, S., Bialecka, M., Huch, M., Kester, L., Van De Wetering, M., Sato, T., Beck, F., Van Oudenaarden, A., Clevers, H., & Deschamps, J. (2014). Transformation of intestinal stem cells into gastric stem cells on loss of transcription factor Cdx2. Nature communications, 5, [6728]. https://doi.org/10.1038/ncomms6728