Transforming growth factor-beta stimulates, and glucocorticoids and epidermal growth factor inhibit brain natriuretic peptide secretion from cultured human amnion cells

Hiroaki Itoh, Norimasa Sagawa, Masaaki Hasegawa, Kumiko Inamori, Hirohisa Ueda, Kyoko Kitagawa, Hidetaka Nanno, Yoshiyuki Ihara, Fuminori Kobayashi, Takahide Mori, Shin Ichi Suga, Takaaki Yoshimasa, Hiroshi Itoh, Kazuwa Nakao

研究成果: Article査読

18 被引用数 (Scopus)

抄録

We previously reported the massive secretion of brain natriuretic peptide (BNP) from human amnion cells and suggested the possible role of BNP in the maintenance of human pregnancy. In this study, to elucidate the regulatory mechanism of BNP secretion from amnion cells, we measured the BNP level in the culture medium of amnion cells by RIA after incubation in the presence of various substances. Among the agents examined, cortisol (1 × 10(-7) to 1 × 10(-6) mol/L), dexamethasone (1 × 10(-8) to 1 × 10(-6) mol/L), and epidermal growth factor (EGF; 2 × 10(-11) to 2 × 10(-8) mol/L) inhibited BNP secretion from the cultured amnion cells in a dose-dependent manner. By contrast, transforming growth factor-β (TGF β; 4 × 10(-11) to 4 × 10(-9) mol/L) caused a 3- to 5-fold increase in BNP secretion. TGF β-augmented BNP secretion was abolished by the addition of cortisol or EGF to the culture medium. Moreover, in this study, we revealed the presence of bioactive TGF β in human amniotic fluid (approximately 4 × 10(-10) mol/L). The present finding of tight regulation of BNP secretion from amnion cells by cortisol, EGF and TGF β, all at the concentrations physiologically present in human amniotic fluid, implies a physiological role of BNP secretion from amnion cells in the pregnant uterus.

本文言語English
ページ(範囲)176-182
ページ数7
ジャーナルJournal of Clinical Endocrinology and Metabolism
79
1
DOI
出版ステータスPublished - 1994 7月
外部発表はい

ASJC Scopus subject areas

  • 内分泌学、糖尿病および代謝内科学
  • 生化学
  • 内分泌学
  • 臨床生化学
  • 生化学、医学

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