Transgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo

Hidetoshi Hasuwa, Yuko Muro, Masahito Ikawa, Noriko Kato, Yoshihide Tsujimoto, Masaru Okabe

研究成果: Comment/debate査読

92 被引用数 (Scopus)

抄録

In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1-Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and oviductal walls when female reproductive tracts were dissected out and placed under excitation light. This characteristic could facilitate examination of sperm migration inside the female reproductive tract as well as facilitating in situ live imaging of the acrosome reaction, the details of which have remained elusive.

本文言語English
ページ(範囲)105-107
ページ数3
ジャーナルExperimental Animals
59
1
DOI
出版ステータスPublished - 2010 3月 11
外部発表はい

ASJC Scopus subject areas

  • 動物科学および動物学
  • 生化学、遺伝学、分子生物学(全般)
  • 獣医学(全般)

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