TY - JOUR
T1 - Transient inhibition of BMP signaling by Noggin induces cardiomyocyte differentiation of mouse embryonic stem cells
AU - Yuasa, Shinsuke
AU - Itabashi, Yuji
AU - Koshimizu, Uichi
AU - Tanaka, Tomofumi
AU - Sugimura, Keijiro
AU - Kinoshita, Masayoshi
AU - Hattori, Fumiyuki
AU - Fukami, Shin Ichi
AU - Shimazaki, Takuya
AU - Okano, Hideyuki
AU - Ogawa, Satoshi
AU - Fukuda, Keiichi
N1 - Funding Information:
This work was (partially) supported by a grant-in-aid from the 21st century Center of Excellence Program of the Ministry of Education, Culture, Sports, Science and Technology, Japan to Keio University. We are grateful to H. Niwa for kindly providing ES cell line EB3 and T. Yoshizaki and Y. Okada for their thoughtful advice and discussion.
PY - 2005
Y1 - 2005
N2 - Embryonic stem (ES) cells are a promising source of cardiomyocytes, but clinical application of ES cells has been hindered by the lack of reliable selective differentiation methods. Differentiation into any lineage is partly dependent on the regulatory mechanisms of normal early development. Although several signals, including bone morphogenetic protein (BMP), Wnt and FGF, are involved in heart development, scarce evidence is available about the exact signals that mediate cardiomyocyte differentiation. While investigating the involvement of BMP signaling in early heart formation in the mouse, we found that the BMP antagonist Noggin is transiently but strongly expressed in the heart-forming region during gastrulation and acts at the level of induction of mesendoderm to establish conditions conducive to cardiogenesis. We applied this finding to develop an effective protocol for obtaining cardiomyocytes from mouse ES cells by inhibition of BMP signaling.
AB - Embryonic stem (ES) cells are a promising source of cardiomyocytes, but clinical application of ES cells has been hindered by the lack of reliable selective differentiation methods. Differentiation into any lineage is partly dependent on the regulatory mechanisms of normal early development. Although several signals, including bone morphogenetic protein (BMP), Wnt and FGF, are involved in heart development, scarce evidence is available about the exact signals that mediate cardiomyocyte differentiation. While investigating the involvement of BMP signaling in early heart formation in the mouse, we found that the BMP antagonist Noggin is transiently but strongly expressed in the heart-forming region during gastrulation and acts at the level of induction of mesendoderm to establish conditions conducive to cardiogenesis. We applied this finding to develop an effective protocol for obtaining cardiomyocytes from mouse ES cells by inhibition of BMP signaling.
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U2 - 10.1038/nbt1093
DO - 10.1038/nbt1093
M3 - Article
C2 - 15867910
AN - SCOPUS:22844439586
SN - 1087-0156
VL - 23
SP - 607
EP - 611
JO - Nature Biotechnology
JF - Nature Biotechnology
IS - 5
ER -