Transient relaxation of plasmid DNA in Escherichia coli by fluoroquinolones

Miki Matsuo, Yoshihisa Ohtsuka, Kazuhiro Kataoka, Tohru Mizushima, Kazuhisa Sekimizu

研究成果: Article査読

4 被引用数 (Scopus)

抄録

We examined the influence of fluoroquinolones (norfloxacin, enoxacin, ofloxacin, levofloxacin, and sparfloxacin) on DNA supercoiling of plasmids in Escherichia coli cells by analysis with agarose gel electrophoresis in the presence of chloroquine. All the fluoroquinolones tested immediately induced DNA relaxation. The relaxed DNA was re-supercoiled, and the process was sensitive to chloramphenicol, suggesting that newly synthesized proteins participate in the reaction. The concentrations of fluoroquinolones required for DNA relaxation were much higher than those required for cell killing. The bactericidal effect of fluoroquinolones is apparently related to mechanisms other than DNA relaxation.

本文言語English
ページ(範囲)985-987
ページ数3
ジャーナルJournal of Pharmacy and Pharmacology
48
9
DOI
出版ステータスPublished - 1996 9

ASJC Scopus subject areas

  • 薬理学
  • 薬科学

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