Transplantation of bulk-separated oligodendrocytes into the brains of shiverer mutant mice: Immunohistochemical and electron microscopic studies on the myelination

Shinichi Kohsaka, Kazunari Yoshida, Yoshiro Inoue, Tomofumi Shinozaki, Hideichi Takayama, Hiroshi Inoue, Katsuhiko Mikoshiba, Kent Takamatsu, Mitsuhiro Otani, Shigeo Toya, Yasuzo Tsukada

研究成果: Article

32 引用 (Scopus)

抜粋

Normal oligodendrocytes were separated from 7-day-old mouse (BALB/c) brains by the Percoll gradient method. Immunohistochemical staining with an anti-galactocerebroside serum revealed that about 85% of the separated cells were oligodendrocytes. The oligodendrocytes were transplanted into the corpus striatum of 4-week-old shiverer mutant mice which are characterized by the lack of myelin basic protein (MBP). Myelination by the implanted oligodendrocytes was investigated immunohistochemically and electron microscopically 6 weeks after operation. Certain areas in the corpus striatum were intensely stained with antiserum to MBP. Electron microscopic examination showed that some axons were surrounded by normal type myelin sheaths with major dense lines. These results clearly indicate that matured oligodendrocytes are able to survive and myelinate the host axons even in the adult brain.

元の言語English
ページ(範囲)137-142
ページ数6
ジャーナルBrain Research
372
発行部数1
DOI
出版物ステータスPublished - 1986 4 30

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

フィンガープリント Transplantation of bulk-separated oligodendrocytes into the brains of shiverer mutant mice: Immunohistochemical and electron microscopic studies on the myelination' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Kohsaka, S., Yoshida, K., Inoue, Y., Shinozaki, T., Takayama, H., Inoue, H., Mikoshiba, K., Takamatsu, K., Otani, M., Toya, S., & Tsukada, Y. (1986). Transplantation of bulk-separated oligodendrocytes into the brains of shiverer mutant mice: Immunohistochemical and electron microscopic studies on the myelination. Brain Research, 372(1), 137-142. https://doi.org/10.1016/0006-8993(86)91466-6