TY - JOUR
T1 - Transplantation of bulk-separated oligodendrocytes into the brains of shiverer mutant mice
T2 - Immunohistochemical and electron microscopic studies on the myelination
AU - Kohsaka, Shinichi
AU - Yoshida, Kazunari
AU - Inoue, Yoshiro
AU - Shinozaki, Tomofumi
AU - Takayama, Hideichi
AU - Inoue, Hiroshi
AU - Mikoshiba, Katsuhiko
AU - Takamatsu, Kent
AU - Otani, Mitsuhiro
AU - Toya, Shigeo
AU - Tsukada, Yasuzo
PY - 1986/4/30
Y1 - 1986/4/30
N2 - Normal oligodendrocytes were separated from 7-day-old mouse (BALB/c) brains by the Percoll gradient method. Immunohistochemical staining with an anti-galactocerebroside serum revealed that about 85% of the separated cells were oligodendrocytes. The oligodendrocytes were transplanted into the corpus striatum of 4-week-old shiverer mutant mice which are characterized by the lack of myelin basic protein (MBP). Myelination by the implanted oligodendrocytes was investigated immunohistochemically and electron microscopically 6 weeks after operation. Certain areas in the corpus striatum were intensely stained with antiserum to MBP. Electron microscopic examination showed that some axons were surrounded by normal type myelin sheaths with major dense lines. These results clearly indicate that matured oligodendrocytes are able to survive and myelinate the host axons even in the adult brain.
AB - Normal oligodendrocytes were separated from 7-day-old mouse (BALB/c) brains by the Percoll gradient method. Immunohistochemical staining with an anti-galactocerebroside serum revealed that about 85% of the separated cells were oligodendrocytes. The oligodendrocytes were transplanted into the corpus striatum of 4-week-old shiverer mutant mice which are characterized by the lack of myelin basic protein (MBP). Myelination by the implanted oligodendrocytes was investigated immunohistochemically and electron microscopically 6 weeks after operation. Certain areas in the corpus striatum were intensely stained with antiserum to MBP. Electron microscopic examination showed that some axons were surrounded by normal type myelin sheaths with major dense lines. These results clearly indicate that matured oligodendrocytes are able to survive and myelinate the host axons even in the adult brain.
KW - major dense line
KW - myelin basic protein
KW - myelination
KW - oligodendrocyte
KW - shiverer mutant mouse
KW - transplantation
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U2 - 10.1016/0006-8993(86)91466-6
DO - 10.1016/0006-8993(86)91466-6
M3 - Article
C2 - 2423188
AN - SCOPUS:0022497740
VL - 372
SP - 137
EP - 142
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1
ER -