Transplantation of mesenchymal stem cells attenuates myocardial injury and dysfunction in a rat model of acute myocarditis

Shunsuke Ohnishi, Bobby Yanagawa, Koichi Tanaka, Yoshinori Miyahara, Hiroaki Obata, Masaharu Kataoka, Makoto Kodama, Hatsue Ishibashi-Ueda, Kenji Kangawa, Soichiro Kitamura, Noritoshi Nagaya

研究成果: Article査読

171 被引用数 (Scopus)

抄録

Acute myocarditis is a non-ischemic inflammatory disease of the myocardium for which there is currently no specific treatment. We have previously shown that mesenchymal stem cells (MSC) can ameliorate heart injury during acute ischemia and in dilated cardiomyopathy; however, the therapeutic potential in acute myocarditis is unclear. In this study, we investigated the ability of MSC to attenuate myocardial injury and dysfunction during the acute phase of experimental myocarditis. Ten-week-old male Lewis rats were injected with porcine myosin to induce myocarditis. Cultured MSC (3 × 106 cells/rat) were injected intravenously 7 days after myosin injection. At 3 weeks, myosin injection resulted in severe inflammation and significant deterioration of cardiac function. MSC transplantation attenuated increases in CD68-positive inflammatory cells and monocyte chemoattractant protein-1 (MCP-1) expression in myocardium, and improved cardiac function in this model. Furthermore, myocardial capillary density was higher in myocarditis tissue, and was further increased by MSC transplantation. In vitro, cultured adult rat cardiomyocytes were injured in response to MCP-1, whereas this effect was attenuated by MSC-derived conditioned medium, suggesting cardioprotective effects of MSC acting in a paracrine manner. MSC transplantation attenuated myocardial injury and dysfunction in a rat model of acute myocarditis, at least in part through paracrine effects of MSC.

本文言語English
ページ(範囲)88-97
ページ数10
ジャーナルJournal of Molecular and Cellular Cardiology
42
1
DOI
出版ステータスPublished - 2007 1月
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 循環器および心血管医学

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