Transplantation of side population cells restores the function of damaged exocrine glands through clusterin

Kenji Mishima, Hiroko Inoue, Tatsuaki Nishiyama, Yo Mabuchi, Yusuke Amano, Fumio Ide, Makoto Matsui, Hiroyuki Yamada, Gou Yamamoto, Junichi Tanaka, Rika Yasuhara, Takashi Sakurai, Masaichi Chang Il Lee, Kan Chiba, Hidetoshi Sumimoto, Yutaka Kawakami, Yumi Matsuzaki, Kazuo Tsubota, Ichiro Saito

研究成果: Article査読

27 被引用数 (Scopus)

抄録

Stem cell-based therapy has been proposed as a promising strategy for regenerating tissues lost through incurable diseases. Side population (SP) cells have been identified as putative stem cells in various organs. To examine therapeutic potential of SP cells in hypofunction of exocrine glands, SP cells isolated from mouse exocrine glands, namely, lacrimal and salivary glands, were transplanted into mice with irradiation-induced hypofunction of the respective glands. The secretions from both glands in the recipient mice were restored within 2 months of transplantation, although the transplanted cells were only sparsely distributed and produced no outgrowths. Consistent with this, most SP cells were shown to be CD31-positive endothelial-like cells. In addition, we clarified that endothelial cell-derived clusterin, a secretory protein, was an essential factor for SP cell-mediated recovery of the hypofunctioning glands because SP cells isolated from salivary glands of clusterin-deficient mice had no therapeutic potential, whereas lentiviral transduction of clusterin restored the hypofunction. In vitro and in vivo studies showed that clusterin had an ability to directly inhibit oxidative stress and oxidative stress-induced cell damage. Thus, endothelial cell-derived clusterin possibly inhibit oxidative stress-induced hypofunction of these glands.

本文言語English
ページ(範囲)1925-1937
ページ数13
ジャーナルStem Cells
30
9
DOI
出版ステータスPublished - 2012 9

ASJC Scopus subject areas

  • 分子医療
  • 発生生物学
  • 細胞生物学

フィンガープリント

「Transplantation of side population cells restores the function of damaged exocrine glands through clusterin」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル