TY - JOUR
T1 - Transporter mRNA expression in a conditionally immortalized rat small intestine epithelial cell line (TR-SIE).
AU - Hosoya, Ken ichi
AU - Tomi, Masatoshi
AU - Takayama, Megumi
AU - Komokata, Yuko
AU - Nakai, Daisuke
AU - Tokui, Taro
AU - Nishimura, Kenji
AU - Ueda, Masatsugu
AU - Obinata, Masuo
AU - Hori, Satoko
AU - Ohtsuki, Sumio
AU - Amidon, Gordon L.
AU - Terasaki, Tetsuya
N1 - Funding Information:
Acknowledgement: This study was supported, in part, by a Grant-in-Aid for Scientiˆc Research from the Japan Society for the Promotion of Science.
PY - 2004/8
Y1 - 2004/8
N2 - Small intestine epithelial cell lines (TR-SIE), which are established from the small intestine of transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen gene (tsA58 Tg rat), were used to characterize the mRNA expression of small intestine transporters. TR-SIE cells had a polygonal morphology and expressed cytokeratin protein and villin mRNA. Although the large T-antigen was strongly expressed at 33 degrees C, this was reduced at 37 and 39 degrees C. Concomitantly, the cell growth was arrested at 37 and 39 degrees C compared with that at 33 degrees C, suggesting that TR-SIE cells are conditionally immortalized cell lines. RT-PCR analysis revealed that TR-SIE cells expressed ABCB1 (mdr1a and mdr1b), ABCB4 (mdr2), ABCC2 (mrp2), ABCC6 (mrp6), ABCG1, ABCG2 (bcrp/mxr), Slc21a7 (Oatp3), Slc15a1 (PepT1), and Slc16a1 (Mct1). Conditionally immortalized rat small intestine epithelial cell lines were established from tsA58 Tg rats and expressed the mRNA of intestinal transporters.
AB - Small intestine epithelial cell lines (TR-SIE), which are established from the small intestine of transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen gene (tsA58 Tg rat), were used to characterize the mRNA expression of small intestine transporters. TR-SIE cells had a polygonal morphology and expressed cytokeratin protein and villin mRNA. Although the large T-antigen was strongly expressed at 33 degrees C, this was reduced at 37 and 39 degrees C. Concomitantly, the cell growth was arrested at 37 and 39 degrees C compared with that at 33 degrees C, suggesting that TR-SIE cells are conditionally immortalized cell lines. RT-PCR analysis revealed that TR-SIE cells expressed ABCB1 (mdr1a and mdr1b), ABCB4 (mdr2), ABCC2 (mrp2), ABCC6 (mrp6), ABCG1, ABCG2 (bcrp/mxr), Slc21a7 (Oatp3), Slc15a1 (PepT1), and Slc16a1 (Mct1). Conditionally immortalized rat small intestine epithelial cell lines were established from tsA58 Tg rats and expressed the mRNA of intestinal transporters.
UR - http://www.scopus.com/inward/record.url?scp=16644386879&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=16644386879&partnerID=8YFLogxK
U2 - 10.2133/dmpk.19.264
DO - 10.2133/dmpk.19.264
M3 - Article
C2 - 15499194
AN - SCOPUS:16644386879
SN - 1347-4367
VL - 19
SP - 264
EP - 269
JO - Drug Metabolism and Pharmacokinetics
JF - Drug Metabolism and Pharmacokinetics
IS - 4
ER -