Transporters on a chip: A fluorescence analysis of an atp-binding cassette (ABC)-transporter

H. Sasaki, Hiroaki Onoe, T. Osaki, R. Kawano, S. Takeuchi

研究成果: Conference contribution

2 引用 (Scopus)

抄録

We have developed a fluorescence-based microfluidic system for high-throughput analyses of the function of ATP-binding cassette (ABC) transporters. Our device consists of polydimethylsiloxane (PDMS) and a glass that tethers vesicles on its surface. Although PDMS is a great material for fabrication of microfluidic channels, it absorbs fluorescent molecules and hampers quantitive fluorescence analyses. We found that conformal deposition of parylenes on the inner surface of PDMS channels dramatically suppressed the absorption of a fluorescent dye, Rhodamine B. In the parylene-coated PDMS channels, immobilized ABC-transporter (MDR1) vesicles exhibited an ATP-dependent transport of a fluorescent substrate, Rhodamine 123.

元の言語English
ホスト出版物のタイトル14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
ページ842-844
ページ数3
2
出版物ステータスPublished - 2010
外部発表Yes
イベント14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
継続期間: 2010 10 32010 10 7

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Netherlands
Groningen
期間10/10/310/10/7

Fingerprint

Polydimethylsiloxane
Fluorescence
Adenosinetriphosphate
Microfluidics
Dyes
Throughput
Fabrication
Glass
Molecules
Substrates

ASJC Scopus subject areas

  • Control and Systems Engineering

これを引用

Sasaki, H., Onoe, H., Osaki, T., Kawano, R., & Takeuchi, S. (2010). Transporters on a chip: A fluorescence analysis of an atp-binding cassette (ABC)-transporter. : 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (巻 2, pp. 842-844)

Transporters on a chip : A fluorescence analysis of an atp-binding cassette (ABC)-transporter. / Sasaki, H.; Onoe, Hiroaki; Osaki, T.; Kawano, R.; Takeuchi, S.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 巻 2 2010. p. 842-844.

研究成果: Conference contribution

Sasaki, H, Onoe, H, Osaki, T, Kawano, R & Takeuchi, S 2010, Transporters on a chip: A fluorescence analysis of an atp-binding cassette (ABC)-transporter. : 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 巻. 2, pp. 842-844, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, Netherlands, 10/10/3.
Sasaki H, Onoe H, Osaki T, Kawano R, Takeuchi S. Transporters on a chip: A fluorescence analysis of an atp-binding cassette (ABC)-transporter. : 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 巻 2. 2010. p. 842-844
Sasaki, H. ; Onoe, Hiroaki ; Osaki, T. ; Kawano, R. ; Takeuchi, S. / Transporters on a chip : A fluorescence analysis of an atp-binding cassette (ABC)-transporter. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 巻 2 2010. pp. 842-844
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AU - Kawano, R.

AU - Takeuchi, S.

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AB - We have developed a fluorescence-based microfluidic system for high-throughput analyses of the function of ATP-binding cassette (ABC) transporters. Our device consists of polydimethylsiloxane (PDMS) and a glass that tethers vesicles on its surface. Although PDMS is a great material for fabrication of microfluidic channels, it absorbs fluorescent molecules and hampers quantitive fluorescence analyses. We found that conformal deposition of parylenes on the inner surface of PDMS channels dramatically suppressed the absorption of a fluorescent dye, Rhodamine B. In the parylene-coated PDMS channels, immobilized ABC-transporter (MDR1) vesicles exhibited an ATP-dependent transport of a fluorescent substrate, Rhodamine 123.

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