Transporters on a chip: A fluorescence analysis of an atp-binding cassette (ABC)-transporter

H. Sasaki, H. Onoe, T. Osaki, R. Kawano, S. Takeuchi

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

We have developed a fluorescence-based microfluidic system for high-throughput analyses of the function of ATP-binding cassette (ABC) transporters. Our device consists of polydimethylsiloxane (PDMS) and a glass that tethers vesicles on its surface. Although PDMS is a great material for fabrication of microfluidic channels, it absorbs fluorescent molecules and hampers quantitive fluorescence analyses. We found that conformal deposition of parylenes on the inner surface of PDMS channels dramatically suppressed the absorption of a fluorescent dye, Rhodamine B. In the parylene-coated PDMS channels, immobilized ABC-transporter (MDR1) vesicles exhibited an ATP-dependent transport of a fluorescent substrate, Rhodamine 123.

本文言語English
ホスト出版物のタイトル14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
ページ842-844
ページ数3
出版ステータスPublished - 2010
外部発表はい
イベント14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
継続期間: 2010 10 32010 10 7

出版物シリーズ

名前14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
2

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/10/310/10/7

ASJC Scopus subject areas

  • Control and Systems Engineering

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