Transportin: Nuclear transport receptor of a novel nuclear protein import pathway

Sara Nakielny, Mikiko C. Siomi, Haruhiko Siomi, W. Matthew Michael, Victoria Pollard, Gideon Dreyfuss

研究成果: Article

84 引用 (Scopus)

抄録

Many nuclear proteins are imported into the cell nucleus by the 'classical' nuclear localization signal (NLS)-mediated import pathway. In this pathway, a sequence rich in basic residues in the protein interacts with a heterodimeric complex termed importin and this, along with the GTPase Ran, mediates nuclear import of the NLS-bearing protein. The heterogeneous nuclear ribonucleoprotein (hnRNP) A1 protein contains a novel nuclear localization sequence, termed M9, that does not contain any clusters of basic residues. Very recently, we showed that M9 directs import into the nucleus by a novel protein import pathway distinct from the classical NLS pathway. A 90- kilodalton protein termed transportin was identified as a protein that specifically interacts with wild-type M9 but not transport-defective M9 mutants. Transportin and an ATP-regenerating system were found to be necessary and sufficient for import of M9-containing proteins in an in vitro import assay. In this report, we provide additional evidence that transportin can interact directly with M9-containing proteins and also show that it can mediate import of full-length hnRNP A1. In addition, Ran, or a Ran-binding protein, is identified as a second protein component of this novel nuclear import pathway. Transportin relatives from Saccharomyces cerevisiae which likely serve as additional nuclear transport receptors are described.

元の言語English
ページ(範囲)261-266
ページ数6
ジャーナルExperimental Cell Research
229
発行部数2
DOI
出版物ステータスPublished - 1996 12 15
外部発表Yes

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Karyopherins
Cell Nucleus Active Transport
Cytoplasmic and Nuclear Receptors
Nuclear Proteins
Nuclear Localization Signals
Proteins
Heterogeneous-Nuclear Ribonucleoproteins
ran GTP-Binding Protein
GTP Phosphohydrolases
Cell Nucleus
Saccharomyces cerevisiae
Signal Transduction
Carrier Proteins
Adenosine Triphosphate

ASJC Scopus subject areas

  • Cell Biology

これを引用

Transportin : Nuclear transport receptor of a novel nuclear protein import pathway. / Nakielny, Sara; Siomi, Mikiko C.; Siomi, Haruhiko; Michael, W. Matthew; Pollard, Victoria; Dreyfuss, Gideon.

:: Experimental Cell Research, 巻 229, 番号 2, 15.12.1996, p. 261-266.

研究成果: Article

Nakielny, S, Siomi, MC, Siomi, H, Michael, WM, Pollard, V & Dreyfuss, G 1996, 'Transportin: Nuclear transport receptor of a novel nuclear protein import pathway', Experimental Cell Research, 巻. 229, 番号 2, pp. 261-266. https://doi.org/10.1006/excr.1996.0369
Nakielny, Sara ; Siomi, Mikiko C. ; Siomi, Haruhiko ; Michael, W. Matthew ; Pollard, Victoria ; Dreyfuss, Gideon. / Transportin : Nuclear transport receptor of a novel nuclear protein import pathway. :: Experimental Cell Research. 1996 ; 巻 229, 番号 2. pp. 261-266.
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