抄録
The importance of androgens (especially 11-ketotestosterone) during previtellogenesis in eels is well established. In wild pubertal migrants, circulating 11-ketotestosterone levels correlate with a number of morphological and molecular changes. Here, we test the prediction that this correlation represents a causal relationship by artificially raising the levels of circulating 11-ketotestosterone in prepubertal nonmigratory female and pubertal, migratory male short-finned eels (Anguilla australis) using sustained-release hormone implants. In females, increases in hepatosomatic index and transcript copy numbers of hepatic apolipoprotein B and microsomal triacylglyceride transfer protein indicated increased repackaging of endogenously sourced triacylglycerides. These changes in liver measures were reflected in increased concentrations of serum triacylglycerides. However, despite a small increase in gonadosomatic index, ovarian lipoprotein receptor transcript abundances were not affected by 11-ketotestosterone. Interestingly, no such changes in hepatic gene expression were detected in a dose-response experiment using males. We propose that the androgens are inducing the observed changes in previtellogenic females, although it remains unclear to what extent these effects are direct or indirect.
| 元の言語 | English |
|---|---|
| ページ（範囲） | R422-R431 |
| ジャーナル | American Journal of Physiology - Heart and Circulatory Physiology |
| 巻 | 310 |
| 発行部数 | 5 |
| DOI | |
| 出版物ステータス | Published - 2016 3 1 |
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ASJC Scopus subject areas
- Medicine(all)
これを引用
Triacylglyceride physiology in the short-finned eel, Anguilla australis--the effects of androgen. / Damsteegt, Erin L.; Ozaki, Yuichi; McCormick, Sally P A; Lokman, P. Mark.
：: American Journal of Physiology - Heart and Circulatory Physiology, 巻 310, 番号 5, 01.03.2016, p. R422-R431.研究成果: Article
}
TY - JOUR
T1 - Triacylglyceride physiology in the short-finned eel, Anguilla australis--the effects of androgen
AU - Damsteegt, Erin L.
AU - Ozaki, Yuichi
AU - McCormick, Sally P A
AU - Lokman, P. Mark
PY - 2016/3/1
Y1 - 2016/3/1
N2 - The importance of androgens (especially 11-ketotestosterone) during previtellogenesis in eels is well established. In wild pubertal migrants, circulating 11-ketotestosterone levels correlate with a number of morphological and molecular changes. Here, we test the prediction that this correlation represents a causal relationship by artificially raising the levels of circulating 11-ketotestosterone in prepubertal nonmigratory female and pubertal, migratory male short-finned eels (Anguilla australis) using sustained-release hormone implants. In females, increases in hepatosomatic index and transcript copy numbers of hepatic apolipoprotein B and microsomal triacylglyceride transfer protein indicated increased repackaging of endogenously sourced triacylglycerides. These changes in liver measures were reflected in increased concentrations of serum triacylglycerides. However, despite a small increase in gonadosomatic index, ovarian lipoprotein receptor transcript abundances were not affected by 11-ketotestosterone. Interestingly, no such changes in hepatic gene expression were detected in a dose-response experiment using males. We propose that the androgens are inducing the observed changes in previtellogenic females, although it remains unclear to what extent these effects are direct or indirect.
AB - The importance of androgens (especially 11-ketotestosterone) during previtellogenesis in eels is well established. In wild pubertal migrants, circulating 11-ketotestosterone levels correlate with a number of morphological and molecular changes. Here, we test the prediction that this correlation represents a causal relationship by artificially raising the levels of circulating 11-ketotestosterone in prepubertal nonmigratory female and pubertal, migratory male short-finned eels (Anguilla australis) using sustained-release hormone implants. In females, increases in hepatosomatic index and transcript copy numbers of hepatic apolipoprotein B and microsomal triacylglyceride transfer protein indicated increased repackaging of endogenously sourced triacylglycerides. These changes in liver measures were reflected in increased concentrations of serum triacylglycerides. However, despite a small increase in gonadosomatic index, ovarian lipoprotein receptor transcript abundances were not affected by 11-ketotestosterone. Interestingly, no such changes in hepatic gene expression were detected in a dose-response experiment using males. We propose that the androgens are inducing the observed changes in previtellogenic females, although it remains unclear to what extent these effects are direct or indirect.
KW - 11-ketotestosterone
KW - androgen receptor
KW - apolipoprotein b
KW - apolipoprotein e
KW - fatty acid transport protein
KW - lipoprotein lipase
KW - low-density lipoprotein receptor
KW - microsomal triacylglyceride transfer protein
KW - time-course experiment
KW - triacylglyceride
KW - very-low density lipoprotein
KW - vitellogenin receptor
UR - http://www.scopus.com/inward/record.url?scp=84971207379&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84971207379&partnerID=8YFLogxK
U2 - 10.1152/ajpregu.00149.2015
DO - 10.1152/ajpregu.00149.2015
M3 - Article
C2 - 26764051
AN - SCOPUS:84971207379
VL - 310
SP - R422-R431
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
SN - 0363-6135
IS - 5
ER -