Turnover rate of tear-film lipid layer determined by fluorophotometry

H. Mochizuki, M. Yamada, S. Hatou, Kazuo Tsubota

研究成果: Article

23 引用 (Scopus)

抄録

Aim: This study was performed to independently assess the turnover rates of aqueous and lipid layers of the tear film. Methods: Two fluorescent dyes, fluorescein sodium and 5-dodecanoylaminofluorescein (DAF), which is a free-fatty-acid conjugate of fluorescein, were applied to the right eye of 12 healthy volunteers. Fluorescent intensity of the precorneal tear film was measured at the central cornea every minute for 10 min for fluorescein sodium, and every 5 min for 50 min for DAF. The turnover rate was calculated by plotting fluorescent intensity against time in a semilog plot and expressed as %/min. Results: Turnover rates of fluorescein sodium and DAF were 10.3 (SD 3.7)%/min and 0.93 (0.36)%/min, respectively. The turnover rate of DAF was significantly lower than that of fluorescein sodium (p<0.05, Mann-Whitney test). The turnover rate of DAF positively correlated with that of fluorescein sodium (r = 0.93, p<0.05). Conclusion: Our results indicate that the turnover of lipids in tears is much slower than the aqueous flow of tears, and that this lipid turnover is associated with the aqueous flow of tears in healthy adults.

元の言語English
ページ(範囲)1535-1538
ページ数4
ジャーナルBritish Journal of Ophthalmology
93
発行部数11
DOI
出版物ステータスPublished - 2009 11

Fingerprint

Fluorophotometry
Fluorescein
Tears
Lipids
Fluorescent Dyes
Nonesterified Fatty Acids
Cornea
Healthy Volunteers
5-dodecanoylaminofluorescein

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

これを引用

Turnover rate of tear-film lipid layer determined by fluorophotometry. / Mochizuki, H.; Yamada, M.; Hatou, S.; Tsubota, Kazuo.

:: British Journal of Ophthalmology, 巻 93, 番号 11, 11.2009, p. 1535-1538.

研究成果: Article

Mochizuki, H. ; Yamada, M. ; Hatou, S. ; Tsubota, Kazuo. / Turnover rate of tear-film lipid layer determined by fluorophotometry. :: British Journal of Ophthalmology. 2009 ; 巻 93, 番号 11. pp. 1535-1538.
@article{20684692e3004fdea5579e8963888b3d,
title = "Turnover rate of tear-film lipid layer determined by fluorophotometry",
abstract = "Aim: This study was performed to independently assess the turnover rates of aqueous and lipid layers of the tear film. Methods: Two fluorescent dyes, fluorescein sodium and 5-dodecanoylaminofluorescein (DAF), which is a free-fatty-acid conjugate of fluorescein, were applied to the right eye of 12 healthy volunteers. Fluorescent intensity of the precorneal tear film was measured at the central cornea every minute for 10 min for fluorescein sodium, and every 5 min for 50 min for DAF. The turnover rate was calculated by plotting fluorescent intensity against time in a semilog plot and expressed as {\%}/min. Results: Turnover rates of fluorescein sodium and DAF were 10.3 (SD 3.7){\%}/min and 0.93 (0.36){\%}/min, respectively. The turnover rate of DAF was significantly lower than that of fluorescein sodium (p<0.05, Mann-Whitney test). The turnover rate of DAF positively correlated with that of fluorescein sodium (r = 0.93, p<0.05). Conclusion: Our results indicate that the turnover of lipids in tears is much slower than the aqueous flow of tears, and that this lipid turnover is associated with the aqueous flow of tears in healthy adults.",
author = "H. Mochizuki and M. Yamada and S. Hatou and Kazuo Tsubota",
year = "2009",
month = "11",
doi = "10.1136/bjo.2008.156828",
language = "English",
volume = "93",
pages = "1535--1538",
journal = "British Journal of Ophthalmology",
issn = "0007-1161",
publisher = "BMJ Publishing Group",
number = "11",

}

TY - JOUR

T1 - Turnover rate of tear-film lipid layer determined by fluorophotometry

AU - Mochizuki, H.

AU - Yamada, M.

AU - Hatou, S.

AU - Tsubota, Kazuo

PY - 2009/11

Y1 - 2009/11

N2 - Aim: This study was performed to independently assess the turnover rates of aqueous and lipid layers of the tear film. Methods: Two fluorescent dyes, fluorescein sodium and 5-dodecanoylaminofluorescein (DAF), which is a free-fatty-acid conjugate of fluorescein, were applied to the right eye of 12 healthy volunteers. Fluorescent intensity of the precorneal tear film was measured at the central cornea every minute for 10 min for fluorescein sodium, and every 5 min for 50 min for DAF. The turnover rate was calculated by plotting fluorescent intensity against time in a semilog plot and expressed as %/min. Results: Turnover rates of fluorescein sodium and DAF were 10.3 (SD 3.7)%/min and 0.93 (0.36)%/min, respectively. The turnover rate of DAF was significantly lower than that of fluorescein sodium (p<0.05, Mann-Whitney test). The turnover rate of DAF positively correlated with that of fluorescein sodium (r = 0.93, p<0.05). Conclusion: Our results indicate that the turnover of lipids in tears is much slower than the aqueous flow of tears, and that this lipid turnover is associated with the aqueous flow of tears in healthy adults.

AB - Aim: This study was performed to independently assess the turnover rates of aqueous and lipid layers of the tear film. Methods: Two fluorescent dyes, fluorescein sodium and 5-dodecanoylaminofluorescein (DAF), which is a free-fatty-acid conjugate of fluorescein, were applied to the right eye of 12 healthy volunteers. Fluorescent intensity of the precorneal tear film was measured at the central cornea every minute for 10 min for fluorescein sodium, and every 5 min for 50 min for DAF. The turnover rate was calculated by plotting fluorescent intensity against time in a semilog plot and expressed as %/min. Results: Turnover rates of fluorescein sodium and DAF were 10.3 (SD 3.7)%/min and 0.93 (0.36)%/min, respectively. The turnover rate of DAF was significantly lower than that of fluorescein sodium (p<0.05, Mann-Whitney test). The turnover rate of DAF positively correlated with that of fluorescein sodium (r = 0.93, p<0.05). Conclusion: Our results indicate that the turnover of lipids in tears is much slower than the aqueous flow of tears, and that this lipid turnover is associated with the aqueous flow of tears in healthy adults.

UR - http://www.scopus.com/inward/record.url?scp=70350436473&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350436473&partnerID=8YFLogxK

U2 - 10.1136/bjo.2008.156828

DO - 10.1136/bjo.2008.156828

M3 - Article

C2 - 19692365

AN - SCOPUS:70350436473

VL - 93

SP - 1535

EP - 1538

JO - British Journal of Ophthalmology

JF - British Journal of Ophthalmology

SN - 0007-1161

IS - 11

ER -