Two different murine monoclonal antibodies B2 and H2 which are specific for human hepatocytes

Toshio Morizane, Kanji Tsuchimoto, Akiko Iino, Shigeru Matsumura, Hidetsugu Saito, Itsurou Satoh, Naoki Kumagai, Yasutaka Inagaiu, Masaharu Tsuchiya, Thomas Brodin, Hans Olov Sjögren

研究成果: Article

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Two different monoclonal antibodies (McAb) designated H2 and B2 which were specific for human hepatocytes were developed by fusing P3-NS1 with spleen or lymphnode cells from BALB/c mice sensitized by a fractionated soluble phase of a human liver homogenate. It was demonstrated by immunoperoxidase technique that the reactivities of these McAb were restricted to human hepatocytes. Twenty four other organs, rat, rabbit livers were negative in staining. The cytoplasm of hepatocytes was stained by the both McAb although the cell membrane was also stained by H2 McAb. These results suggest that the antigens defined by these are different from LSP which is known to be species-nonspecific and localized exclusively on cell membrane and other liver-specific antigens already known. It is proposed that the antigen defined by H2 McAb is designated human liver-specific antigen 1 (HLSA1) and that the antigen defined by B2 McAb is designated human liver-specific antigen 2 (HLSA2).

元の言語English
ページ(範囲)726-735
ページ数10
ジャーナルKanzo
27
発行部数6
DOI
出版物ステータスPublished - 1986

ASJC Scopus subject areas

  • Hepatology

フィンガープリント Two different murine monoclonal antibodies B2 and H2 which are specific for human hepatocytes' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

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    Morizane, T., Tsuchimoto, K., Iino, A., Matsumura, S., Saito, H., Satoh, I., Kumagai, N., Inagaiu, Y., Tsuchiya, M., Brodin, T., & Sjögren, H. O. (1986). Two different murine monoclonal antibodies B2 and H2 which are specific for human hepatocytes. Kanzo, 27(6), 726-735. https://doi.org/10.2957/kanzo.27.726