Two dimensional sequential cell assembly by sliding a parylene filter

K. Inoue; K. Kamiya; Yuta Abe; T. Osaki; Norihisa Miki; S. Takeuchi

Two dimensional sequential cell assembly by sliding a parylene filter

K. Inoue, K. Kamiya, Yuta Abe, T. Osaki, Norihisa Miki, S. Takeuchi

研究成果: Conference contribution

抄録

We demonstrated the two-dimensional assembly of cell clusters in a plane by sliding parylene filter with cell-trapping sites. Gently trapping cells in a designated order while maintaining the contact between them is necessary to investigate cell-cell interactions. Therefore, we developed the mechanical cell trapping system using a parylene filter which was slid to sequentially trap cells in a straight line and control the order. In this study, by using the cell-trapping system, we succeeded in assembling the two-dimensional cell clusters with a cross-shape and an elephant-shape. This system is readily applicable to analysis cell-cell interaction in biological tissue.

本文言語	English
ホスト出版物のタイトル	MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
出版社	Chemical and Biological Microsystems Society
ページ	508-509
ページ数	2
ISBN（電子版）	9780979806483
出版ステータス	Published - 2015
イベント	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of 継続期間: 2015 10 25 → 2015 10 29

Other

Other	19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Country	Korea, Republic of
City	Gyeongju
Period	15/10/25 → 15/10/29

ASJC Scopus subject areas

Control and Systems Engineering

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引用スタイル

Inoue, K, Kamiya, K, Abe, Y, Osaki, T, Miki, N & Takeuchi, S 2015, Two dimensional sequential cell assembly by sliding a parylene filter. in MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 508-509, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, Gyeongju, Korea, Republic of, 15/10/25.

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abstract = "We demonstrated the two-dimensional assembly of cell clusters in a plane by sliding parylene filter with cell-trapping sites. Gently trapping cells in a designated order while maintaining the contact between them is necessary to investigate cell-cell interactions. Therefore, we developed the mechanical cell trapping system using a parylene filter which was slid to sequentially trap cells in a straight line and control the order. In this study, by using the cell-trapping system, we succeeded in assembling the two-dimensional cell clusters with a cross-shape and an elephant-shape. This system is readily applicable to analysis cell-cell interaction in biological tissue.",

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language = "English",

pages = "508--509",

booktitle = "MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

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AU - Inoue, K.

AU - Kamiya, K.

AU - Abe, Yuta

AU - Osaki, T.

AU - Miki, Norihisa

AU - Takeuchi, S.

PY - 2015

Y1 - 2015

N2 - We demonstrated the two-dimensional assembly of cell clusters in a plane by sliding parylene filter with cell-trapping sites. Gently trapping cells in a designated order while maintaining the contact between them is necessary to investigate cell-cell interactions. Therefore, we developed the mechanical cell trapping system using a parylene filter which was slid to sequentially trap cells in a straight line and control the order. In this study, by using the cell-trapping system, we succeeded in assembling the two-dimensional cell clusters with a cross-shape and an elephant-shape. This system is readily applicable to analysis cell-cell interaction in biological tissue.

AB - We demonstrated the two-dimensional assembly of cell clusters in a plane by sliding parylene filter with cell-trapping sites. Gently trapping cells in a designated order while maintaining the contact between them is necessary to investigate cell-cell interactions. Therefore, we developed the mechanical cell trapping system using a parylene filter which was slid to sequentially trap cells in a straight line and control the order. In this study, by using the cell-trapping system, we succeeded in assembling the two-dimensional cell clusters with a cross-shape and an elephant-shape. This system is readily applicable to analysis cell-cell interaction in biological tissue.

KW - Biological tissue

KW - Cell

KW - Membrane protein

KW - Microfluidic device

KW - Photolithography

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T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015

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