Two novel phytotoxic substances from Leucas aspera

A. K M Mominul Islam, Osamu Ohno, Kiyotake Suenaga, Hisashi Kato-Noguchi

研究成果: Article

9 引用 (Scopus)

抄録

Leucas aspera (Lamiaceae), an aromatic herbaceous plant, is well known for many medicinal properties and a number of bioactive compounds against animal cells have been isolated. However, phytotoxic substances from L. aspera have not yet been documented in the literature. Therefore, current research was conducted to explore the phytotoxic properties and substances in L. aspera. Aqueous methanol extracts of L. aspera inhibited the germination and growth of garden cress (Lepidum sativum) and barnyard grass (Echinochloa crus-galli), and the inhibitory activities were concentration dependent. These results suggest that the plant may have phytotoxic substances. The extracts were then purified by several chromatographic runs. The final purification was achieved by reversed-phase HPLC to give an equilibrium (or inseparable) 3:2 mixture of two labdane type diterpenes (compounds 1 and 2). These compounds were characterized as (rel 5S,6R,8R,9R,10S,13S,15S,16R)-6-acetoxy-9,13;15,16-diepoxy-15-hydroxy-16-methoxylabdane (1) and (rel 5S,6R,8R,9R,10S,13S,15R,16R)-6-acetoxy-9,13;15,16-diepoxy-15-hydroxy-16-methoxylabdane (2) by spectroscopic analyses. A mixture of the two compounds inhibits the germination and seedling growth of garden cress and barnyard grass at concentrations greater than 30 and 3μM, respectively. The concentration required for 50% growth inhibition (I50) of the test species ranges from 31 to 80μM, which suggests that the mixture of these compounds, are responsible for the phytotoxic activity of L. aspera plant extract.

元の言語English
ページ(範囲)877-883
ページ数7
ジャーナルJournal of Plant Physiology
171
発行部数11
DOI
出版物ステータスPublished - 2014 7 1

Fingerprint

Leucas aspera
Echinochloa
Lepidium sativum
Echinochloa crus-galli
Germination
Growth
Lamiaceae
Diterpenes
Plant Extracts
Seedlings
Methanol
germination
High Pressure Liquid Chromatography
medicinal properties
essential oil crops
extracts
herbaceous plants
reversed-phase high performance liquid chromatography
diterpenoids
plant extracts

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science
  • Medicine(all)

これを引用

Two novel phytotoxic substances from Leucas aspera. / Islam, A. K M Mominul; Ohno, Osamu; Suenaga, Kiyotake; Kato-Noguchi, Hisashi.

:: Journal of Plant Physiology, 巻 171, 番号 11, 01.07.2014, p. 877-883.

研究成果: Article

Islam, A. K M Mominul ; Ohno, Osamu ; Suenaga, Kiyotake ; Kato-Noguchi, Hisashi. / Two novel phytotoxic substances from Leucas aspera. :: Journal of Plant Physiology. 2014 ; 巻 171, 番号 11. pp. 877-883.
@article{cf82e8e57cbf4785b838be88640aec3e,
title = "Two novel phytotoxic substances from Leucas aspera",
abstract = "Leucas aspera (Lamiaceae), an aromatic herbaceous plant, is well known for many medicinal properties and a number of bioactive compounds against animal cells have been isolated. However, phytotoxic substances from L. aspera have not yet been documented in the literature. Therefore, current research was conducted to explore the phytotoxic properties and substances in L. aspera. Aqueous methanol extracts of L. aspera inhibited the germination and growth of garden cress (Lepidum sativum) and barnyard grass (Echinochloa crus-galli), and the inhibitory activities were concentration dependent. These results suggest that the plant may have phytotoxic substances. The extracts were then purified by several chromatographic runs. The final purification was achieved by reversed-phase HPLC to give an equilibrium (or inseparable) 3:2 mixture of two labdane type diterpenes (compounds 1 and 2). These compounds were characterized as (rel 5S,6R,8R,9R,10S,13S,15S,16R)-6-acetoxy-9,13;15,16-diepoxy-15-hydroxy-16-methoxylabdane (1) and (rel 5S,6R,8R,9R,10S,13S,15R,16R)-6-acetoxy-9,13;15,16-diepoxy-15-hydroxy-16-methoxylabdane (2) by spectroscopic analyses. A mixture of the two compounds inhibits the germination and seedling growth of garden cress and barnyard grass at concentrations greater than 30 and 3μM, respectively. The concentration required for 50{\%} growth inhibition (I50) of the test species ranges from 31 to 80μM, which suggests that the mixture of these compounds, are responsible for the phytotoxic activity of L. aspera plant extract.",
keywords = "Bioactive compounds, Diterpene, Lamiaceae, Medicinal plant, Plant growth inhibitor",
author = "Islam, {A. K M Mominul} and Osamu Ohno and Kiyotake Suenaga and Hisashi Kato-Noguchi",
year = "2014",
month = "7",
day = "1",
doi = "10.1016/j.jplph.2014.03.003",
language = "English",
volume = "171",
pages = "877--883",
journal = "Journal of Plant Physiology",
issn = "0176-1617",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "11",

}

TY - JOUR

T1 - Two novel phytotoxic substances from Leucas aspera

AU - Islam, A. K M Mominul

AU - Ohno, Osamu

AU - Suenaga, Kiyotake

AU - Kato-Noguchi, Hisashi

PY - 2014/7/1

Y1 - 2014/7/1

N2 - Leucas aspera (Lamiaceae), an aromatic herbaceous plant, is well known for many medicinal properties and a number of bioactive compounds against animal cells have been isolated. However, phytotoxic substances from L. aspera have not yet been documented in the literature. Therefore, current research was conducted to explore the phytotoxic properties and substances in L. aspera. Aqueous methanol extracts of L. aspera inhibited the germination and growth of garden cress (Lepidum sativum) and barnyard grass (Echinochloa crus-galli), and the inhibitory activities were concentration dependent. These results suggest that the plant may have phytotoxic substances. The extracts were then purified by several chromatographic runs. The final purification was achieved by reversed-phase HPLC to give an equilibrium (or inseparable) 3:2 mixture of two labdane type diterpenes (compounds 1 and 2). These compounds were characterized as (rel 5S,6R,8R,9R,10S,13S,15S,16R)-6-acetoxy-9,13;15,16-diepoxy-15-hydroxy-16-methoxylabdane (1) and (rel 5S,6R,8R,9R,10S,13S,15R,16R)-6-acetoxy-9,13;15,16-diepoxy-15-hydroxy-16-methoxylabdane (2) by spectroscopic analyses. A mixture of the two compounds inhibits the germination and seedling growth of garden cress and barnyard grass at concentrations greater than 30 and 3μM, respectively. The concentration required for 50% growth inhibition (I50) of the test species ranges from 31 to 80μM, which suggests that the mixture of these compounds, are responsible for the phytotoxic activity of L. aspera plant extract.

AB - Leucas aspera (Lamiaceae), an aromatic herbaceous plant, is well known for many medicinal properties and a number of bioactive compounds against animal cells have been isolated. However, phytotoxic substances from L. aspera have not yet been documented in the literature. Therefore, current research was conducted to explore the phytotoxic properties and substances in L. aspera. Aqueous methanol extracts of L. aspera inhibited the germination and growth of garden cress (Lepidum sativum) and barnyard grass (Echinochloa crus-galli), and the inhibitory activities were concentration dependent. These results suggest that the plant may have phytotoxic substances. The extracts were then purified by several chromatographic runs. The final purification was achieved by reversed-phase HPLC to give an equilibrium (or inseparable) 3:2 mixture of two labdane type diterpenes (compounds 1 and 2). These compounds were characterized as (rel 5S,6R,8R,9R,10S,13S,15S,16R)-6-acetoxy-9,13;15,16-diepoxy-15-hydroxy-16-methoxylabdane (1) and (rel 5S,6R,8R,9R,10S,13S,15R,16R)-6-acetoxy-9,13;15,16-diepoxy-15-hydroxy-16-methoxylabdane (2) by spectroscopic analyses. A mixture of the two compounds inhibits the germination and seedling growth of garden cress and barnyard grass at concentrations greater than 30 and 3μM, respectively. The concentration required for 50% growth inhibition (I50) of the test species ranges from 31 to 80μM, which suggests that the mixture of these compounds, are responsible for the phytotoxic activity of L. aspera plant extract.

KW - Bioactive compounds

KW - Diterpene

KW - Lamiaceae

KW - Medicinal plant

KW - Plant growth inhibitor

UR - http://www.scopus.com/inward/record.url?scp=84901008796&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901008796&partnerID=8YFLogxK

U2 - 10.1016/j.jplph.2014.03.003

DO - 10.1016/j.jplph.2014.03.003

M3 - Article

C2 - 24913044

AN - SCOPUS:84901008796

VL - 171

SP - 877

EP - 883

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

SN - 0176-1617

IS - 11

ER -