Tyrosine phosphorylation of paxillin α is involved in temporospatial regulation of paxillin-containing focal adhesion formation and F-actin organization in motile cells

Kuniaki Nakamura, Hajime Yano, Hiroshi Uchida, Shigeru Hashimoto, Erik Schaefer, Hisataka Sabe

研究成果: Article

99 引用 (Scopus)

抄録

Temporal and spatial regulation of actin-based cytoskeletal organization and focal adhesion formation play an essential role in cell migration. Here, we show that tyrosine phosphorylation of a focal adhesion protein, paxillin, crucially participates in these regulations. We found that tyrosine phosphorylation of paxillin was a prominent event upon integrin activation during epithelial-mesenchymal trans-differentiation and cell migration. Four major tyrosine phosphorylation sites were identified, and two of them were highly inducible upon integrin activation. Paxillin exhibits three distinct subcellular localizations as follows: localization along the cell periphery colocalized with circumferential actin meshworks, macroaggregation at focal adhesions connected to actin stress fibers, and diffuse cytoplasmic distribution. Tyrosine phosphorylation of paxillin localized at the cell periphery and focal adhesions was shown using phosphorylation site-specific antibodies. Mutations in the phosphorylation sites affected the peripheral localization of paxillin and paxillin-containing focal adhesion formation during cell migration and cell-cell collision, accompanied by altered actin organizations. Our analysis indicates that phosphorylation of multiple tyrosines in paxillin α is necessary for the proper function of paxillin and is involved in the temporospatial regulation of focal adhesion formation and actin cytoskeletal organization in motile cells.

元の言語English
ページ(範囲)27155-27164
ページ数10
ジャーナルJournal of Biological Chemistry
275
発行部数35
DOI
出版物ステータスPublished - 2000 9 1
外部発表Yes

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Paxillin
Phosphorylation
Focal Adhesions
Tyrosine
Actins
Adhesion
Cell Movement
Integrins
Chemical activation
Stress Fibers
Cell Adhesion
Organizations
Mutation
Antibodies
Fibers

ASJC Scopus subject areas

  • Biochemistry

これを引用

Tyrosine phosphorylation of paxillin α is involved in temporospatial regulation of paxillin-containing focal adhesion formation and F-actin organization in motile cells. / Nakamura, Kuniaki; Yano, Hajime; Uchida, Hiroshi; Hashimoto, Shigeru; Schaefer, Erik; Sabe, Hisataka.

:: Journal of Biological Chemistry, 巻 275, 番号 35, 01.09.2000, p. 27155-27164.

研究成果: Article

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AU - Yano, Hajime

AU - Uchida, Hiroshi

AU - Hashimoto, Shigeru

AU - Schaefer, Erik

AU - Sabe, Hisataka

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