Ultraprecision cutting of single-crystal calcium fluoride for fabricating micro flow cell for protein structure analysis

Ryo Komiya, Tetsunari Kimura, Minoru Kubo, Jiwang Yan

研究成果: Paper査読

抄録

In infrared spectroscopic analysis of the structures of protein crystals, a micro-flow cell made of single crystal calcium fluoride (CaF2) is needed. In this research, fly cutting of micro grooves on a CaF2 substrate was conducted using a single-crystal diamond tool. The effects of cutting direction, up/down cut, and tool rake angle on ductile-brittle transition of cutting behavior were investigated. Based on the experimental results and analysis, optimal cutting conditions were selected which enabled ductile machining of CaF2. As a result, a 10 μm deep micro-flow cell was successfully fabricated with surface roughness of 2.4 nmRa. The availability of the flow cell was confirmed by visible absorption spectroscopy of protein.

本文言語English
出版ステータスPublished - 2015 10月 18
イベント8th International Conference on Leading Edge Manufacturing in 21st Century, LEM 2015 - Kyoto, Japan
継続期間: 2015 10月 182015 10月 22

Other

Other8th International Conference on Leading Edge Manufacturing in 21st Century, LEM 2015
国/地域Japan
CityKyoto
Period15/10/1815/10/22

ASJC Scopus subject areas

  • 産業および生産工学

フィンガープリント

「Ultraprecision cutting of single-crystal calcium fluoride for fabricating micro flow cell for protein structure analysis」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル