Ultrasound-induced DNA damage and signal transductions indicated by gammaH2AX

Yukihiro Furusawa, Yoshisada Fujiwara, Qing Li Zhao, Mariame Ali Hassan, Ryohei Ogawa, Yoshiaki Tabuchi, Ichiro Takasaki, Akihisa Takahashi, Takeo Ohnishi, Takashi Kondo

研究成果: Conference contribution

2 引用 (Scopus)

抜粋

Ultrasound (US) has been shown to induce cancer cell death via different forms including apoptosis. Here, we report the potential of low-intensity pulsed US (LIPUS) to induce genomic DNA damage and subsequent DNA damage response. Using the ionizing radiation-induced DNA doublestrand breaks (DSBs) as the positive control, we were able to observe the induction of DSBs (as neutral comet tails) and the subsequent formation of gammaH2AX-positive foci (by immunofluorescence detection) in human leukemia cells following exposure to LIPUS. The LIPUS-induced DNA damage arose most likely from the mechanical, but not sonochemical, effect of cavitation, based on our observation that the suppression of inertial cavitation abrogated the gammH2AX foci formation, whereas scavenging of free radical formation (e.g., hydroxyl radical) had no protective effect on it. Treatment with the specific kinase inhibitor of ATM or DNA-PKcs, which can phosphorylate H2AX Ser139, revealed that US-induced gammaH2AX was inhibited more effectively by the DNA-PK inhibitor than ATM kinase inhibitor. Notably, these inhibitor effects were opposite to those with radiation-induced gammH2AX. In conclusion, we report, for the first time that US can induce DNA damage and the DNA damage response as indicated by gammaH2AX was triggered by the cavitational mechanical effects. Thus, it is expected that the data shown here may provide a better understanding of the cellular responses to US.

元の言語English
ホスト出版物のタイトル10th International Symposium on Therapeutic Ultrasound, ISTU 2010
ページ322-325
ページ数4
DOI
出版物ステータスPublished - 2011
外部発表Yes
イベント10th International Symposium on Therapeutic Ultrasound, ISTU 2010 - Tokyo, Japan
継続期間: 2010 6 92010 6 12

出版物シリーズ

名前AIP Conference Proceedings
1359
ISSN(印刷物)0094-243X
ISSN(電子版)1551-7616

Other

Other10th International Symposium on Therapeutic Ultrasound, ISTU 2010
Japan
Tokyo
期間10/6/910/6/12

ASJC Scopus subject areas

  • Physics and Astronomy(all)

フィンガープリント Ultrasound-induced DNA damage and signal transductions indicated by gammaH2AX' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Furusawa, Y., Fujiwara, Y., Zhao, Q. L., Hassan, M. A., Ogawa, R., Tabuchi, Y., Takasaki, I., Takahashi, A., Ohnishi, T., & Kondo, T. (2011). Ultrasound-induced DNA damage and signal transductions indicated by gammaH2AX. : 10th International Symposium on Therapeutic Ultrasound, ISTU 2010 (pp. 322-325). (AIP Conference Proceedings; 巻数 1359). https://doi.org/10.1063/1.3607926