Ultrastructural analysis of the paranodal junction of myelinated fibers in 31-month-old-rats

Ichiro Sugiyama, Kayoko Tanaka, Masumi Akita, Kazunari Yoshida, Takeshi Kawase, Hiroaki Asou

研究成果: Article

27 引用 (Scopus)

抄録

Recent studies have revealed a significant decrease in white matter volume, including loss of myelin, with age but minimal decrease in gray matter volume (Guttmann et al., [1998] Neurology 50:972-978). Myelin is necessary for the rapid conduction of impulses along axons. Myelinated nerve includes various domains, the node of Ranvier, the paranodal region, the juxtaparanodal region and the internode. The paranodal junction may serve to anchor the myelin sheath to the axon. We analyzed the ultrastructure of the paranodal region in myelinated fibers from the aged rat brain. Severe alterations of myelinated fibers were observed in 31-month-old rats, resulting in the appearance of macrophages, splitting of the myelin sheath, myelin balloon formation and separation from the axon. Many paranodal retractions of myelinated axons occurred in the aged rats. It should be noted that the paranodal junction is functionally important, serving to anchor the myelin to the axon and that there is a diffusion barrier in the paranodal region. We analyzed myelin-related proteins from young and aged rat brains. The 21.5-kDa isoform of myelin basic protein (MBP) almost disappeared in the 31-month-old rats, whereas other myelin proteins were not significantly changed between young and aged rats. These results suggest that this isoform, a highly cationic charged major dense component protein that binds lipid bilayer in the membrane, may participate in the formation of a paranodal diffusion barrier at the myelin/noncompact membrane border.

元の言語English
ページ(範囲)309-317
ページ数9
ジャーナルJournal of Neuroscience Research
70
発行部数3
DOI
出版物ステータスPublished - 2002 11 2

Fingerprint

Myelin Sheath
Axons
Myelin Proteins
Ranvier's Nodes
Myelin Basic Protein
Membranes
Brain
Lipid Bilayers
Neurology
Protein Isoforms
Macrophages
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

これを引用

Ultrastructural analysis of the paranodal junction of myelinated fibers in 31-month-old-rats. / Sugiyama, Ichiro; Tanaka, Kayoko; Akita, Masumi; Yoshida, Kazunari; Kawase, Takeshi; Asou, Hiroaki.

:: Journal of Neuroscience Research, 巻 70, 番号 3, 02.11.2002, p. 309-317.

研究成果: Article

Sugiyama, Ichiro ; Tanaka, Kayoko ; Akita, Masumi ; Yoshida, Kazunari ; Kawase, Takeshi ; Asou, Hiroaki. / Ultrastructural analysis of the paranodal junction of myelinated fibers in 31-month-old-rats. :: Journal of Neuroscience Research. 2002 ; 巻 70, 番号 3. pp. 309-317.
@article{b9d011e9e4574d8ab95f6b22e36a606e,
title = "Ultrastructural analysis of the paranodal junction of myelinated fibers in 31-month-old-rats",
abstract = "Recent studies have revealed a significant decrease in white matter volume, including loss of myelin, with age but minimal decrease in gray matter volume (Guttmann et al., [1998] Neurology 50:972-978). Myelin is necessary for the rapid conduction of impulses along axons. Myelinated nerve includes various domains, the node of Ranvier, the paranodal region, the juxtaparanodal region and the internode. The paranodal junction may serve to anchor the myelin sheath to the axon. We analyzed the ultrastructure of the paranodal region in myelinated fibers from the aged rat brain. Severe alterations of myelinated fibers were observed in 31-month-old rats, resulting in the appearance of macrophages, splitting of the myelin sheath, myelin balloon formation and separation from the axon. Many paranodal retractions of myelinated axons occurred in the aged rats. It should be noted that the paranodal junction is functionally important, serving to anchor the myelin to the axon and that there is a diffusion barrier in the paranodal region. We analyzed myelin-related proteins from young and aged rat brains. The 21.5-kDa isoform of myelin basic protein (MBP) almost disappeared in the 31-month-old rats, whereas other myelin proteins were not significantly changed between young and aged rats. These results suggest that this isoform, a highly cationic charged major dense component protein that binds lipid bilayer in the membrane, may participate in the formation of a paranodal diffusion barrier at the myelin/noncompact membrane border.",
keywords = "21.5-kDa MBP, Myelin balloon formation, Paranodal loops, Splitting of myelin sheath",
author = "Ichiro Sugiyama and Kayoko Tanaka and Masumi Akita and Kazunari Yoshida and Takeshi Kawase and Hiroaki Asou",
year = "2002",
month = "11",
day = "2",
doi = "10.1002/jnr.10386",
language = "English",
volume = "70",
pages = "309--317",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Ultrastructural analysis of the paranodal junction of myelinated fibers in 31-month-old-rats

AU - Sugiyama, Ichiro

AU - Tanaka, Kayoko

AU - Akita, Masumi

AU - Yoshida, Kazunari

AU - Kawase, Takeshi

AU - Asou, Hiroaki

PY - 2002/11/2

Y1 - 2002/11/2

N2 - Recent studies have revealed a significant decrease in white matter volume, including loss of myelin, with age but minimal decrease in gray matter volume (Guttmann et al., [1998] Neurology 50:972-978). Myelin is necessary for the rapid conduction of impulses along axons. Myelinated nerve includes various domains, the node of Ranvier, the paranodal region, the juxtaparanodal region and the internode. The paranodal junction may serve to anchor the myelin sheath to the axon. We analyzed the ultrastructure of the paranodal region in myelinated fibers from the aged rat brain. Severe alterations of myelinated fibers were observed in 31-month-old rats, resulting in the appearance of macrophages, splitting of the myelin sheath, myelin balloon formation and separation from the axon. Many paranodal retractions of myelinated axons occurred in the aged rats. It should be noted that the paranodal junction is functionally important, serving to anchor the myelin to the axon and that there is a diffusion barrier in the paranodal region. We analyzed myelin-related proteins from young and aged rat brains. The 21.5-kDa isoform of myelin basic protein (MBP) almost disappeared in the 31-month-old rats, whereas other myelin proteins were not significantly changed between young and aged rats. These results suggest that this isoform, a highly cationic charged major dense component protein that binds lipid bilayer in the membrane, may participate in the formation of a paranodal diffusion barrier at the myelin/noncompact membrane border.

AB - Recent studies have revealed a significant decrease in white matter volume, including loss of myelin, with age but minimal decrease in gray matter volume (Guttmann et al., [1998] Neurology 50:972-978). Myelin is necessary for the rapid conduction of impulses along axons. Myelinated nerve includes various domains, the node of Ranvier, the paranodal region, the juxtaparanodal region and the internode. The paranodal junction may serve to anchor the myelin sheath to the axon. We analyzed the ultrastructure of the paranodal region in myelinated fibers from the aged rat brain. Severe alterations of myelinated fibers were observed in 31-month-old rats, resulting in the appearance of macrophages, splitting of the myelin sheath, myelin balloon formation and separation from the axon. Many paranodal retractions of myelinated axons occurred in the aged rats. It should be noted that the paranodal junction is functionally important, serving to anchor the myelin to the axon and that there is a diffusion barrier in the paranodal region. We analyzed myelin-related proteins from young and aged rat brains. The 21.5-kDa isoform of myelin basic protein (MBP) almost disappeared in the 31-month-old rats, whereas other myelin proteins were not significantly changed between young and aged rats. These results suggest that this isoform, a highly cationic charged major dense component protein that binds lipid bilayer in the membrane, may participate in the formation of a paranodal diffusion barrier at the myelin/noncompact membrane border.

KW - 21.5-kDa MBP

KW - Myelin balloon formation

KW - Paranodal loops

KW - Splitting of myelin sheath

UR - http://www.scopus.com/inward/record.url?scp=0037010302&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037010302&partnerID=8YFLogxK

U2 - 10.1002/jnr.10386

DO - 10.1002/jnr.10386

M3 - Article

C2 - 12391590

AN - SCOPUS:0037010302

VL - 70

SP - 309

EP - 317

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 3

ER -