Undesigned selection for replication termination of bacterial chromosomes

Nobuaki Kono, Kazuharu Arakawa, Mitsuru Sato, Hirofumi Yoshikawa, Masaru Tomita, Mitsuhiro Itaya

研究成果: Article

3 引用 (Scopus)

抄録

The oriC DNA replication origin in bacterial chromosomes, the location of which appears to be physically identified, is genetically regulated by relevant molecular machinery. In contrast, the location of the terminus remains obscure for many bacterial replicons, except for terC, the proposed and well-studied chromosome termination site in certain bacteria. The terC locus, which is composed of specific sequences for its binding protein, is located at a site opposite from oriC, exhibiting a symmetric structure around the oriC-terC axis. Here, we investigated Bacillus subtilis 168 strains whose axes were hindered and found that the native terC function was robust. However, eradication of terminus region specific binding protein resulted in the natural terC sites not being used for termination; instead, new termini were selected at a location exactly opposite to oriC. We concluded that replication generally terminates at the loci where the two approaching replisomes meet. This site was automatically selected, and two replisomes moving at the same rate supported symmetrical chromosome structures relative to oriC. The rule, which was even validated by artificial chromosomes irrespective of oriC, should be general for replicons administered by two replisomes.

元の言語English
ページ(範囲)2918-2927
ページ数10
ジャーナルJournal of Molecular Biology
426
発行部数16
DOI
出版物ステータスPublished - 2014 8 12

Fingerprint

Bacterial Chromosomes
Replicon
Carrier Proteins
Artificial Chromosomes
Chromosome Structures
Replication Origin
Bacillus subtilis
DNA Replication
Chromosomes
Bacteria

ASJC Scopus subject areas

  • Molecular Biology

これを引用

Undesigned selection for replication termination of bacterial chromosomes. / Kono, Nobuaki; Arakawa, Kazuharu; Sato, Mitsuru; Yoshikawa, Hirofumi; Tomita, Masaru; Itaya, Mitsuhiro.

:: Journal of Molecular Biology, 巻 426, 番号 16, 12.08.2014, p. 2918-2927.

研究成果: Article

Kono, Nobuaki ; Arakawa, Kazuharu ; Sato, Mitsuru ; Yoshikawa, Hirofumi ; Tomita, Masaru ; Itaya, Mitsuhiro. / Undesigned selection for replication termination of bacterial chromosomes. :: Journal of Molecular Biology. 2014 ; 巻 426, 番号 16. pp. 2918-2927.
@article{aece018d02ce40168b1c74823a9c22c8,
title = "Undesigned selection for replication termination of bacterial chromosomes",
abstract = "The oriC DNA replication origin in bacterial chromosomes, the location of which appears to be physically identified, is genetically regulated by relevant molecular machinery. In contrast, the location of the terminus remains obscure for many bacterial replicons, except for terC, the proposed and well-studied chromosome termination site in certain bacteria. The terC locus, which is composed of specific sequences for its binding protein, is located at a site opposite from oriC, exhibiting a symmetric structure around the oriC-terC axis. Here, we investigated Bacillus subtilis 168 strains whose axes were hindered and found that the native terC function was robust. However, eradication of terminus region specific binding protein resulted in the natural terC sites not being used for termination; instead, new termini were selected at a location exactly opposite to oriC. We concluded that replication generally terminates at the loci where the two approaching replisomes meet. This site was automatically selected, and two replisomes moving at the same rate supported symmetrical chromosome structures relative to oriC. The rule, which was even validated by artificial chromosomes irrespective of oriC, should be general for replicons administered by two replisomes.",
keywords = "Bacillus subtilis, circular chromosome, genomic structure, high-throughput sequencing, replication terminus",
author = "Nobuaki Kono and Kazuharu Arakawa and Mitsuru Sato and Hirofumi Yoshikawa and Masaru Tomita and Mitsuhiro Itaya",
year = "2014",
month = "8",
day = "12",
doi = "10.1016/j.jmb.2014.06.005",
language = "English",
volume = "426",
pages = "2918--2927",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "16",

}

TY - JOUR

T1 - Undesigned selection for replication termination of bacterial chromosomes

AU - Kono, Nobuaki

AU - Arakawa, Kazuharu

AU - Sato, Mitsuru

AU - Yoshikawa, Hirofumi

AU - Tomita, Masaru

AU - Itaya, Mitsuhiro

PY - 2014/8/12

Y1 - 2014/8/12

N2 - The oriC DNA replication origin in bacterial chromosomes, the location of which appears to be physically identified, is genetically regulated by relevant molecular machinery. In contrast, the location of the terminus remains obscure for many bacterial replicons, except for terC, the proposed and well-studied chromosome termination site in certain bacteria. The terC locus, which is composed of specific sequences for its binding protein, is located at a site opposite from oriC, exhibiting a symmetric structure around the oriC-terC axis. Here, we investigated Bacillus subtilis 168 strains whose axes were hindered and found that the native terC function was robust. However, eradication of terminus region specific binding protein resulted in the natural terC sites not being used for termination; instead, new termini were selected at a location exactly opposite to oriC. We concluded that replication generally terminates at the loci where the two approaching replisomes meet. This site was automatically selected, and two replisomes moving at the same rate supported symmetrical chromosome structures relative to oriC. The rule, which was even validated by artificial chromosomes irrespective of oriC, should be general for replicons administered by two replisomes.

AB - The oriC DNA replication origin in bacterial chromosomes, the location of which appears to be physically identified, is genetically regulated by relevant molecular machinery. In contrast, the location of the terminus remains obscure for many bacterial replicons, except for terC, the proposed and well-studied chromosome termination site in certain bacteria. The terC locus, which is composed of specific sequences for its binding protein, is located at a site opposite from oriC, exhibiting a symmetric structure around the oriC-terC axis. Here, we investigated Bacillus subtilis 168 strains whose axes were hindered and found that the native terC function was robust. However, eradication of terminus region specific binding protein resulted in the natural terC sites not being used for termination; instead, new termini were selected at a location exactly opposite to oriC. We concluded that replication generally terminates at the loci where the two approaching replisomes meet. This site was automatically selected, and two replisomes moving at the same rate supported symmetrical chromosome structures relative to oriC. The rule, which was even validated by artificial chromosomes irrespective of oriC, should be general for replicons administered by two replisomes.

KW - Bacillus subtilis

KW - circular chromosome

KW - genomic structure

KW - high-throughput sequencing

KW - replication terminus

UR - http://www.scopus.com/inward/record.url?scp=84904742106&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904742106&partnerID=8YFLogxK

U2 - 10.1016/j.jmb.2014.06.005

DO - 10.1016/j.jmb.2014.06.005

M3 - Article

C2 - 24946150

AN - SCOPUS:84904742106

VL - 426

SP - 2918

EP - 2927

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 16

ER -