Using encapsulation to improve the viability of cryopreserved cells

Yoshifumi Matsumoto, Yukihiro Morinaga, Masanobu Ujihira, Kotaro Oka, Kazuo Tanishita

研究成果: Chapter

1 引用 (Scopus)

抄録

The purpose of this study is to clarify whether encapsulated cells have an advantage over suspended cells in cryopreservation. Rat pheochromocytoma (PC12) cells were selected for test biological cell and microencapsulated in alginate-polylysine-alginate membranes. Microencapsulated PC12 cells were frozen with differential scanning calorimetry (DSC) at a cooling rate of 0.5 to 10°C/min, their latent heat was measured among the freezing process over the temperature range 4 to -80°C. Their post-thaw viability were evaluated by dye exclusion assay and dopamine release. As a result, latent heat of encapsulated cells was lower than that of suspended cells at a cooling rate of 0.5 and 1°C/min. This is because extra-capsule was frozen and intra-capsule unfrozen, as ice crystals forms in extra-capsule space. Post-thaw viability of microencapsulated PC12 cells was improved at 0.5 and 1°C/min compared with that of suspended cells. Therefore, in microencapsulated PC12 cells, achievement of intra-capsule unfrozen condition during freezing leads to reducing the solution effect and improving the viability.

元の言語English
ホスト出版物のタイトルAmerican Society of Mechanical Engineers, Bioengineering Division (Publication) BED
出版者ASME
ページ57-58
ページ数2
44
ISBN(印刷物)0791816435
出版物ステータスPublished - 1999
イベントAdvances in Heat and Mass Transfer in Biotechnology - 1999 (The ASME International Mechanical Engineering Congress and Exposition) - Nashville, TN, USA
継続期間: 1999 11 141999 11 19

Other

OtherAdvances in Heat and Mass Transfer in Biotechnology - 1999 (The ASME International Mechanical Engineering Congress and Exposition)
Nashville, TN, USA
期間99/11/1499/11/19

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Latent heat
Alginate
Encapsulation
Freezing
Cells
Cooling
Ice
Rats
Differential scanning calorimetry
Assays
Dyes
Membranes
Crystals
Temperature
Dopamine

ASJC Scopus subject areas

  • Engineering(all)

これを引用

Matsumoto, Y., Morinaga, Y., Ujihira, M., Oka, K., & Tanishita, K. (1999). Using encapsulation to improve the viability of cryopreserved cells. : American Society of Mechanical Engineers, Bioengineering Division (Publication) BED (巻 44, pp. 57-58). ASME.

Using encapsulation to improve the viability of cryopreserved cells. / Matsumoto, Yoshifumi; Morinaga, Yukihiro; Ujihira, Masanobu; Oka, Kotaro; Tanishita, Kazuo.

American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. 巻 44 ASME, 1999. p. 57-58.

研究成果: Chapter

Matsumoto, Y, Morinaga, Y, Ujihira, M, Oka, K & Tanishita, K 1999, Using encapsulation to improve the viability of cryopreserved cells. : American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. 巻. 44, ASME, pp. 57-58, Advances in Heat and Mass Transfer in Biotechnology - 1999 (The ASME International Mechanical Engineering Congress and Exposition), Nashville, TN, USA, 99/11/14.
Matsumoto Y, Morinaga Y, Ujihira M, Oka K, Tanishita K. Using encapsulation to improve the viability of cryopreserved cells. : American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. 巻 44. ASME. 1999. p. 57-58
Matsumoto, Yoshifumi ; Morinaga, Yukihiro ; Ujihira, Masanobu ; Oka, Kotaro ; Tanishita, Kazuo. / Using encapsulation to improve the viability of cryopreserved cells. American Society of Mechanical Engineers, Bioengineering Division (Publication) BED. 巻 44 ASME, 1999. pp. 57-58
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