USING ENCAPSULATION TO IMPROVE THE VIABILITY OF CRYOPRESERVED CELLS

Yoshifumi Matsumoto, Yukihiro Morinaga, Masanobu Ujihira, Kotaro Oka, Kazuo Tanishita

研究成果: Conference contribution

抄録

The purpose of this study is to clarify whether encapsulated cells have an advantage over suspended cells in cryopreservation. Rat pheochromocytoma (PC 12) cells were selected for test biological cell and microencapsulated in alginate-polylysine-alginate membranes. Microencapsulated PC12 cells were frozen with differential scanning calorimetry (DSC) at a cooling rate of 0.5 to 10°C/min, their latent heat was measured among the freezing process over the temperature range 4 to -80°C. Their post-thaw viability were evaluated by dye exclusion assay and dopamine release. As a result, latent heat of encapsulated cells was lower than that of suspended cells at a cooling rate of 0.5 and 1°C/min. This is because extra-capsule was frozen and intra-capsule unfrozen, as ice crystals forms in extra-capsule space. Post-thaw viability of microencapsulated PC 12 cells was improved at 0.5 and 1°C/min compared with that of suspended cells. Therefore, in microencapsulated PC 12 cells, achievement of intra-capsule unfrozen condition during freezing leads to reducing the solution effect and improving the viability.

本文言語English
ホスト出版物のタイトルAdvances in Heat and Mass Transfer in Biotechnology
出版社American Society of Mechanical Engineers (ASME)
ページ57-58
ページ数2
ISBN(電子版)9780791816431
DOI
出版ステータスPublished - 1999
イベントASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999 - Nashville, United States
継続期間: 1999 11月 141999 11月 19

出版物シリーズ

名前ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
1999-E

Conference

ConferenceASME 1999 International Mechanical Engineering Congress and Exposition, IMECE 1999
国/地域United States
CityNashville
Period99/11/1499/11/19

ASJC Scopus subject areas

  • 機械工学

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