Using piggyBac transposon gene expression vectors to transfect Zscan5b gene into mouse pluripotent stem cells

Mitsutoshi Yamada, Tohru Sugawara, Shinju Usami, Rina Nakanishi, Hidenori Akustu

研究成果: Article査読

1 被引用数 (Scopus)

抄録

We recently demonstrated that the zygotic gene Zscan5b is involved in maintaining the genomic integrity of pluripotent stem cells (PSCs) during mitosis and that Zscan5b gene expression can prevent chromosomal abnormalities. Here, we provide a detailed protocol for the transfection of mouse embryonic stem cells (mESCs) and mouse-induced PSCs (miPSCs) with a Zscan5b constitutive expression plasmid, using the piggyBac transposon gene expression vector system. For complete details on the use and execution of this protocol, please refer to Ogawa et al. (2019).

本文言語English
論文番号100811
ジャーナルSTAR Protocols
2
3
DOI
出版ステータスPublished - 2021 9月 17

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 神経科学(全般)
  • 免疫学および微生物学(全般)
  • 医学(全般)

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