V642I APP-inducible neuronal cells: A model system for investigating Alzheimer's disorders

Takako Niikura, Norie Murayama, Yu Ichi Hashimoto, Yuko Ito, Yohichi Yamagishi, Masaaki Matsuoka, Yuji Takeuchi, Sadakazu Aiso, Ikuo Nishimoto

研究成果: Article査読

26 被引用数 (Scopus)

抄録

APP is a precursor of β amyloid deposited in Alzheimer's disease (AD). Although genetic studies established that mutations in APP cause familial AD (FAD), the mechanism for neuronal death by FAD mutants has not been well understood. We established neuronal cells (F11/EcR/V642I cells) in which V642I APP was inducibly expressed by ecdysone. Treatment with ecdysone, but not vehicle, killed most cells within a few days, with rounding, shrinkage, and detachment as well as nuclear fragmentation. Death was suppressed by Ac-DEVD-CHO and pertussis toxin. Electron microscopic analysis revealed that apoptosis occurred in ecdysone-treated cells. V642I-APP-induced death was suppressed by the anti-AD factors estrogen and apoE2. These data demonstrate not only that expression of this FAD gene causes neuronal apoptosis, but that F11/EcR/V642I cells, the first neuronal cells with inducible FAD gene expression, provide a useful model system in investigating AD disorders. (C) 2000 Academic Press.

本文言語English
ページ(範囲)445-454
ページ数10
ジャーナルBiochemical and Biophysical Research Communications
274
2
DOI
出版ステータスPublished - 2000 8 2

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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