Visualization and quantification of cerebral metabolic fluxes of glucose in awake mice

研究成果: Article

32 引用 (Scopus)

抜粋

Biotransformation of glucose in organs includes multiple pathways, while quantitative evaluation of percentages of its utilization for individual pathways and their spatial heterogeneity in vivo remain unknown. Imaging MS (IMS) and metabolomics combined with a focused microwave irradiation for rapidly fixing tissue metabolism allowed us to quantify and visualize metabolic fluxes of glucose-derived metabolites in the mouse brain in vivo. At 15 min after the intraperitoneal injection of 13C6-labeled glucose, the mouse brain was exposed to focused microwave irradiation, which can stop brain metabolism within 1 s. Quantification of metabolic intermediates containing 13C atoms revealed that a majority of the 13C6-glucose was diverted into syntheses of glutamate, lactate, and uridine diphosphate (UDP)-glucose. IMS showed that regions rich in glutaminergic neurons exhibited a large signal of 13C2-labeled glutamate. On the other hand, the midbrain region was enriched with an intensive 13C6-labeled UDP-glucose signal, suggesting an active glycogen synthesis. Collectively, application of the current method makes it possible to examine the fluxes of glucose metabolism in a region-specific manner.

元の言語English
ページ(範囲)829-838
ページ数10
ジャーナルProteomics
14
発行部数7-8
DOI
出版物ステータスPublished - 2014

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

フィンガープリント Visualization and quantification of cerebral metabolic fluxes of glucose in awake mice' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用