Visualization of oxidative processes at the cellular level during neutrophil-mediated cytotoxicity against a human hepatoma cell line, HCC-M

Hidetsugu Saito, D. Fukumura, I. Kurose, M. Suematsu, S. Tada, T. Kagawa, S. Miura, T. Morizane, M. Tsuchiya

研究成果: Article

23 引用 (Scopus)

抄録

Human neutrophil-mediated oxidative processes against a human hepatoma cell line, HCC-M, was visualized at the cellular level by using a silicon-intensified target camera and subsequently processing with a computer-assisted digital imaging processor. Neutrophils were activated by a streptococcal preparation, OK-432. A hydroperoxide-sensitive tracer, dichlorofluorescin diacetate, was loaded in HCC-M and temporal and spatial changes of lipid peroxides in this cell after addition of stimulated neutrophils were analyzed. The luminol-dependent chemiluminescence activity of neutrophils was significantly enhanced and continued for at least 2 hr by stimulation with OK-432, and its activity was shown to be accumulated at the site where a neutrophil attached with HCC-M. The intensity of dichlorofluorescein fluorescence in HCC-M rapidly increased after adding stimulated neutrophils, and their reaction was significantly attenuated by superoxide dismutase. The number of non-viable cells was increased as the dichlorofluorescein fluorescence increased. It is suggested that oxidative stress may play an important role in neutrophil-mediated tumor-cell damage.

元の言語English
ページ(範囲)124-129
ページ数6
ジャーナルInternational Journal of Cancer
51
発行部数1
DOI
出版物ステータスPublished - 1992

Fingerprint

Hepatocellular Carcinoma
Neutrophils
Cell Line
Picibanil
Fluorescence
Luminol
Lipid Peroxides
Silicon
Luminescence
Hydrogen Peroxide
Superoxide Dismutase
Oxidative Stress
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

これを引用

Visualization of oxidative processes at the cellular level during neutrophil-mediated cytotoxicity against a human hepatoma cell line, HCC-M. / Saito, Hidetsugu; Fukumura, D.; Kurose, I.; Suematsu, M.; Tada, S.; Kagawa, T.; Miura, S.; Morizane, T.; Tsuchiya, M.

:: International Journal of Cancer, 巻 51, 番号 1, 1992, p. 124-129.

研究成果: Article

Saito, Hidetsugu ; Fukumura, D. ; Kurose, I. ; Suematsu, M. ; Tada, S. ; Kagawa, T. ; Miura, S. ; Morizane, T. ; Tsuchiya, M. / Visualization of oxidative processes at the cellular level during neutrophil-mediated cytotoxicity against a human hepatoma cell line, HCC-M. :: International Journal of Cancer. 1992 ; 巻 51, 番号 1. pp. 124-129.
@article{7a453fd06600473c8c3af5c9450b1b89,
title = "Visualization of oxidative processes at the cellular level during neutrophil-mediated cytotoxicity against a human hepatoma cell line, HCC-M",
abstract = "Human neutrophil-mediated oxidative processes against a human hepatoma cell line, HCC-M, was visualized at the cellular level by using a silicon-intensified target camera and subsequently processing with a computer-assisted digital imaging processor. Neutrophils were activated by a streptococcal preparation, OK-432. A hydroperoxide-sensitive tracer, dichlorofluorescin diacetate, was loaded in HCC-M and temporal and spatial changes of lipid peroxides in this cell after addition of stimulated neutrophils were analyzed. The luminol-dependent chemiluminescence activity of neutrophils was significantly enhanced and continued for at least 2 hr by stimulation with OK-432, and its activity was shown to be accumulated at the site where a neutrophil attached with HCC-M. The intensity of dichlorofluorescein fluorescence in HCC-M rapidly increased after adding stimulated neutrophils, and their reaction was significantly attenuated by superoxide dismutase. The number of non-viable cells was increased as the dichlorofluorescein fluorescence increased. It is suggested that oxidative stress may play an important role in neutrophil-mediated tumor-cell damage.",
author = "Hidetsugu Saito and D. Fukumura and I. Kurose and M. Suematsu and S. Tada and T. Kagawa and S. Miura and T. Morizane and M. Tsuchiya",
year = "1992",
doi = "10.1002/ijc.2910510122",
language = "English",
volume = "51",
pages = "124--129",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Visualization of oxidative processes at the cellular level during neutrophil-mediated cytotoxicity against a human hepatoma cell line, HCC-M

AU - Saito, Hidetsugu

AU - Fukumura, D.

AU - Kurose, I.

AU - Suematsu, M.

AU - Tada, S.

AU - Kagawa, T.

AU - Miura, S.

AU - Morizane, T.

AU - Tsuchiya, M.

PY - 1992

Y1 - 1992

N2 - Human neutrophil-mediated oxidative processes against a human hepatoma cell line, HCC-M, was visualized at the cellular level by using a silicon-intensified target camera and subsequently processing with a computer-assisted digital imaging processor. Neutrophils were activated by a streptococcal preparation, OK-432. A hydroperoxide-sensitive tracer, dichlorofluorescin diacetate, was loaded in HCC-M and temporal and spatial changes of lipid peroxides in this cell after addition of stimulated neutrophils were analyzed. The luminol-dependent chemiluminescence activity of neutrophils was significantly enhanced and continued for at least 2 hr by stimulation with OK-432, and its activity was shown to be accumulated at the site where a neutrophil attached with HCC-M. The intensity of dichlorofluorescein fluorescence in HCC-M rapidly increased after adding stimulated neutrophils, and their reaction was significantly attenuated by superoxide dismutase. The number of non-viable cells was increased as the dichlorofluorescein fluorescence increased. It is suggested that oxidative stress may play an important role in neutrophil-mediated tumor-cell damage.

AB - Human neutrophil-mediated oxidative processes against a human hepatoma cell line, HCC-M, was visualized at the cellular level by using a silicon-intensified target camera and subsequently processing with a computer-assisted digital imaging processor. Neutrophils were activated by a streptococcal preparation, OK-432. A hydroperoxide-sensitive tracer, dichlorofluorescin diacetate, was loaded in HCC-M and temporal and spatial changes of lipid peroxides in this cell after addition of stimulated neutrophils were analyzed. The luminol-dependent chemiluminescence activity of neutrophils was significantly enhanced and continued for at least 2 hr by stimulation with OK-432, and its activity was shown to be accumulated at the site where a neutrophil attached with HCC-M. The intensity of dichlorofluorescein fluorescence in HCC-M rapidly increased after adding stimulated neutrophils, and their reaction was significantly attenuated by superoxide dismutase. The number of non-viable cells was increased as the dichlorofluorescein fluorescence increased. It is suggested that oxidative stress may play an important role in neutrophil-mediated tumor-cell damage.

UR - http://www.scopus.com/inward/record.url?scp=0026562568&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026562568&partnerID=8YFLogxK

U2 - 10.1002/ijc.2910510122

DO - 10.1002/ijc.2910510122

M3 - Article

C2 - 1314229

AN - SCOPUS:0026562568

VL - 51

SP - 124

EP - 129

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 1

ER -