Water-soluble NIR fluorescent probes based on squaraine and their application for protein labeling

Keitaro Umezawa, Daniel Citterio, Koji Suzuki

研究成果: Article査読

55 被引用数 (Scopus)

抄録

Water-soluble near-infrared (NIR) fluorescent labeling probes, named KSQ-3 and -4, which are based on a squaraine backbone, were synthesized and applied to biological labeling. The presented results demonstrate that the large, planar and hydrophobic squaraine dye becomes fully soluble in aqueous solution by the introduction of several sulfo group terminated alkyl substituents. Especially KSQ-4, which is substituted with four sulfo groups, exhibited perfect water solubility and significant fluorescence emission at the NIR region (817 nm) in the presence of bovine serum albumin (BSA). BSA was covalently labeled with KSQ-4, and the conjugate showed a strong absorption peak at 787 nm, which indicates compatibility with commercially available NIR laser diodes used for exciting the fluorophore. Furthermore, strong fluorescence emission was observed at 812 nm (φ = 0.08). 2008

本文言語English
ページ(範囲)213-217
ページ数5
ジャーナルanalytical sciences
24
2
DOI
出版ステータスPublished - 2008 2月

ASJC Scopus subject areas

  • 分析化学

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