Xanthurenic acid is an endogenous substrate for the silkworm cytosolic sulfotransferase, bmST1

Akira Kushida, Ryo Horie, Kenji Hattori, Hiroshi Hamamoto, Kazuhisa Sekimizu, Hiroomi Tamura

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Sulfotransferase enzymes are known to regulate physiologically active substances such as steroids and catecholamines in mammals. Although invertebrates also express sulfotransferases, their biological function is mostly unclear. In a previous study, we reported that 4-nitrocatechol and the gallete ester are substrates for the silkworm sulfotransferase bmST1. The Km of bmST1 for these substrates is high. However, endogenous substrates of bmST1 have not yet been determined. We therefore investigated endogenous bmST1 substrates and carried out a detailed expression profile analysis of bmST1. We found that xanthurenic acid, a tryptophan metabolite, is a possible endogenous substrate of bmST1. The Km of bmST1 for xanthurenic acid is low, in the μM range, which is lower than that for previously reported substrates. Additionally, xanthurenic acid is a tryptophan metabolite that characteristically shows toxicity in vivo. High dose administration of xanthurenic acid resulted in inhibition of cuticular biosynthesis. The expression of the bmST1 gene reached a maximal level in the Malpighian tubule at the 4th molting stage, when amino acid metabolism might be activated. Our results suggest that bmST1 plays a role in detoxification of xanthurenic acid in the silkworm.

本文言語English
ページ(範囲)83-88
ページ数6
ジャーナルJournal of Insect Physiology
58
1
DOI
出版ステータスPublished - 2012 1

ASJC Scopus subject areas

  • 生理学
  • 昆虫科学

フィンガープリント

「Xanthurenic acid is an endogenous substrate for the silkworm cytosolic sulfotransferase, bmST1」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル