Yb integrates piRNA intermediates and processing factors into perinuclear bodies to enhance piRISC assembly

Yukiko Murota, Hirotsugu Ishizu, Shinichi Nakagawa, Yuka W. Iwasaki, Shinsuke Shibata, Miharu K. Kamatani, Kuniaki Saito, Hideyuki Okano, Haruhiko Siomi, Mikiko C. Siomi

研究成果: Article査読

53 被引用数 (Scopus)

抄録

PIWI-interacting RNAs (piRNAs) direct Piwi to repress transposons and maintain genome integrity in Drosophila ovarian somatic cells. piRNA maturation and association with Piwi occur at perinuclear Yb bodies, the centers of piRNA biogenesis. Here, we show that piRNA intermediates arising from the piRNA cluster flamenco ( flam) localize to perinuclear foci adjacent to Yb bodies, termed Flam bodies. RNAi-based screening of piRNA factors revealed that Flam body formation depends on Yb, the core component of Yb bodies, while Piwi and another Yb body component, Armitage, are dispensable forformation. Abolishing the RNA-binding activity of Yb disrupts both Flam bodies and Yb bodies. Yb directly binds flam, but not transcripts from neighboring protein-coding genes. Thus, Yb integrates piRNA intermediates and piRNA processing factors selectively into Flam bodies and Yb bodies, respectively. We suggest that Yb is a key upstream factor in the cytoplasmic phase of the piRNA pathway in ovarian somatic cells.

本文言語English
ページ(範囲)103-113
ページ数11
ジャーナルCell Reports
8
1
DOI
出版ステータスPublished - 2014 7 10

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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