Zanamivir immobilized magnetic beads for voltammetric measurement of neuraminidase at gold-modified boron doped diamond electrode

Wulan Tri Wahyuni, Tribidasari A. Ivandini, Endang Saepudin, Yasuaki Einaga

研究成果: Conference contribution

抄録

Biomolecule modified magnetic beads has been widely used in separation and sensing process. This study used streptavidin modified magnetic beads to immobilize biotin modified zanamivir. Biotin-streptavidin affinity facilitates immobilization of zanamivir on magnetic beads. Then interaction of zanamivir and neuraminidase was adopted as basic for enzyme detection. Detection of neuraminidase was performed at gold modified BDD using cyclic voltammetry technique. The measurement was carried out based on alteration of electrochemical signals of working electrode as neuraminidase response. The result showed that zanamivir was successfully immobilized on magnetic beads. The optimum amount of magnetic beads for zanamivir immobilization was 120 ug. Linear responses of neuraminidase were detected in concentration range of 0-15 mU. Detection limit (LOD) of measurement was 2.32 mU (R2 = 0.959) with precision as % RSD of 1.41%. Measurement of neuraminidase on magnetic beads could be also performed in the presence of mucin matrix. The linearity range was 0-8 mU with LOD of 0.64 mU (R2 = 0.950) and % RSD of 7.25%.

本文言語English
ホスト出版物のタイトルInternational Symposium on Current Progress in Mathematics and Sciences 2015, ISCPMS 2015: Proceedings of the 1st International Symposium on Current Progress in Mathematics and Sciences
出版社American Institute of Physics Inc.
1729
ISBN(電子版)9780735413764
DOI
出版ステータスPublished - 2016 4月 19
イベント1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015 - Depok, Indonesia
継続期間: 2015 11月 32015 11月 4

Other

Other1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015
国/地域Indonesia
CityDepok
Period15/11/315/11/4

ASJC Scopus subject areas

  • 物理学および天文学(全般)

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