Zonal responsiveness of the human intervertebral disc to bone morphogenetic protein-2

Hyang Kim, Jeong Uk Lee, Seong Hwan Moon, Hyung Chan Kim, Un Hae Kwon, Nam Hun Seol, Ho Joong Kim, Jin Oh Park, Heoung Jae Chun, Il Keun Kwon, Hwan Mo Lee

研究成果: Article

30 引用 (Scopus)

抄録

Study Design.: In vitro experiment using bone morphogenetic protein-2 (BMP-2) and cells from the nucleus pulposus (NP), transitional zone (TZ), and anulus fibrosus (AF) of the human intervertebral disc (IVD). Objective.: To demonstrate the differential effect of BMP-2 on DNA synthesis, proteoglycan synthesis, and osteocalcin mRNA expression in human IVD cells from the NP, TZ, and AF, respectively. Summary of Background Data.: BMP-2 has been proven to be effective in stimulating proteoglycan synthesis in articular chondrocytes and IVD cells from the NP. Nevertheless, the effect of BMP-2 on cells from different regions of the IVD has not yet been thoroughly elucidated. Methods.: Human IVDs were harvested from surgical disc procedures and tissue from the NP, TZ, and AF was obtained. Disc tissue was enzymatically digested, and IVD cells were cultured three-dimensionally in alginate beads. Then IVD cell cultures from the NP, TZ, and AF were exposed to BMP-2. DNA synthesis and newly synthesized proteoglycan were measured. Reverse transcription-polymerase chain reaction for mRNA expression of bone sialoprotein, DLX5, osteocalcin, and collagen type I, was performed. Results.: Cells from the AF responded to BMP-2 with mitogenesis. There was no significant increase in DNA synthesis in cultures from the NP and TZ treated with BMP-2. Only cells from the NP showed a significant increase in newly synthesized proteoglycan in response to BMP-2. IVD cells from all zones demonstrated no significant expression of bone sialoprotein, DLX5, osteocalcin mRNA after treatment with BMP-2. Conclusion.: BMP-2 clearly exerted a mitogenic effect on AF cells, and stimulated proteoglycan synthesis in NP cells. However, BMP-2 did not have an osteogenic effect in any IVD region. Taken together, these results confirm that BMP-2 can be used as an anabolic agent for mitogenesis in AF cells and NP cell matrix regeneration without the possibility of osteogenesis.

元の言語English
ページ(範囲)1834-1838
ページ数5
ジャーナルSpine
34
発行部数17
DOI
出版物ステータスPublished - 2009 8 1
外部発表Yes

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Bone Morphogenetic Protein 2
Intervertebral Disc
Cell Nucleus
Proteoglycans
Osteocalcin
Integrin-Binding Sialoprotein
Messenger RNA
DNA
Nucleus Pulposus
Anabolic Agents
Chondrocytes
Collagen Type I
Annulus Fibrosus
Osteogenesis
Reverse Transcription
Regeneration
Cultured Cells
Cell Culture Techniques
Joints

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Clinical Neurology

これを引用

Kim, H., Lee, J. U., Moon, S. H., Kim, H. C., Kwon, U. H., Seol, N. H., ... Lee, H. M. (2009). Zonal responsiveness of the human intervertebral disc to bone morphogenetic protein-2. Spine, 34(17), 1834-1838. https://doi.org/10.1097/BRS.0b013e3181ae18ba

Zonal responsiveness of the human intervertebral disc to bone morphogenetic protein-2. / Kim, Hyang; Lee, Jeong Uk; Moon, Seong Hwan; Kim, Hyung Chan; Kwon, Un Hae; Seol, Nam Hun; Kim, Ho Joong; Park, Jin Oh; Chun, Heoung Jae; Kwon, Il Keun; Lee, Hwan Mo.

:: Spine, 巻 34, 番号 17, 01.08.2009, p. 1834-1838.

研究成果: Article

Kim, H, Lee, JU, Moon, SH, Kim, HC, Kwon, UH, Seol, NH, Kim, HJ, Park, JO, Chun, HJ, Kwon, IK & Lee, HM 2009, 'Zonal responsiveness of the human intervertebral disc to bone morphogenetic protein-2', Spine, 巻. 34, 番号 17, pp. 1834-1838. https://doi.org/10.1097/BRS.0b013e3181ae18ba
Kim H, Lee JU, Moon SH, Kim HC, Kwon UH, Seol NH その他. Zonal responsiveness of the human intervertebral disc to bone morphogenetic protein-2. Spine. 2009 8 1;34(17):1834-1838. https://doi.org/10.1097/BRS.0b013e3181ae18ba
Kim, Hyang ; Lee, Jeong Uk ; Moon, Seong Hwan ; Kim, Hyung Chan ; Kwon, Un Hae ; Seol, Nam Hun ; Kim, Ho Joong ; Park, Jin Oh ; Chun, Heoung Jae ; Kwon, Il Keun ; Lee, Hwan Mo. / Zonal responsiveness of the human intervertebral disc to bone morphogenetic protein-2. :: Spine. 2009 ; 巻 34, 番号 17. pp. 1834-1838.
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abstract = "Study Design.: In vitro experiment using bone morphogenetic protein-2 (BMP-2) and cells from the nucleus pulposus (NP), transitional zone (TZ), and anulus fibrosus (AF) of the human intervertebral disc (IVD). Objective.: To demonstrate the differential effect of BMP-2 on DNA synthesis, proteoglycan synthesis, and osteocalcin mRNA expression in human IVD cells from the NP, TZ, and AF, respectively. Summary of Background Data.: BMP-2 has been proven to be effective in stimulating proteoglycan synthesis in articular chondrocytes and IVD cells from the NP. Nevertheless, the effect of BMP-2 on cells from different regions of the IVD has not yet been thoroughly elucidated. Methods.: Human IVDs were harvested from surgical disc procedures and tissue from the NP, TZ, and AF was obtained. Disc tissue was enzymatically digested, and IVD cells were cultured three-dimensionally in alginate beads. Then IVD cell cultures from the NP, TZ, and AF were exposed to BMP-2. DNA synthesis and newly synthesized proteoglycan were measured. Reverse transcription-polymerase chain reaction for mRNA expression of bone sialoprotein, DLX5, osteocalcin, and collagen type I, was performed. Results.: Cells from the AF responded to BMP-2 with mitogenesis. There was no significant increase in DNA synthesis in cultures from the NP and TZ treated with BMP-2. Only cells from the NP showed a significant increase in newly synthesized proteoglycan in response to BMP-2. IVD cells from all zones demonstrated no significant expression of bone sialoprotein, DLX5, osteocalcin mRNA after treatment with BMP-2. Conclusion.: BMP-2 clearly exerted a mitogenic effect on AF cells, and stimulated proteoglycan synthesis in NP cells. However, BMP-2 did not have an osteogenic effect in any IVD region. Taken together, these results confirm that BMP-2 can be used as an anabolic agent for mitogenesis in AF cells and NP cell matrix regeneration without the possibility of osteogenesis.",
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AU - Lee, Jeong Uk

AU - Moon, Seong Hwan

AU - Kim, Hyung Chan

AU - Kwon, Un Hae

AU - Seol, Nam Hun

AU - Kim, Ho Joong

AU - Park, Jin Oh

AU - Chun, Heoung Jae

AU - Kwon, Il Keun

AU - Lee, Hwan Mo

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N2 - Study Design.: In vitro experiment using bone morphogenetic protein-2 (BMP-2) and cells from the nucleus pulposus (NP), transitional zone (TZ), and anulus fibrosus (AF) of the human intervertebral disc (IVD). Objective.: To demonstrate the differential effect of BMP-2 on DNA synthesis, proteoglycan synthesis, and osteocalcin mRNA expression in human IVD cells from the NP, TZ, and AF, respectively. Summary of Background Data.: BMP-2 has been proven to be effective in stimulating proteoglycan synthesis in articular chondrocytes and IVD cells from the NP. Nevertheless, the effect of BMP-2 on cells from different regions of the IVD has not yet been thoroughly elucidated. Methods.: Human IVDs were harvested from surgical disc procedures and tissue from the NP, TZ, and AF was obtained. Disc tissue was enzymatically digested, and IVD cells were cultured three-dimensionally in alginate beads. Then IVD cell cultures from the NP, TZ, and AF were exposed to BMP-2. DNA synthesis and newly synthesized proteoglycan were measured. Reverse transcription-polymerase chain reaction for mRNA expression of bone sialoprotein, DLX5, osteocalcin, and collagen type I, was performed. Results.: Cells from the AF responded to BMP-2 with mitogenesis. There was no significant increase in DNA synthesis in cultures from the NP and TZ treated with BMP-2. Only cells from the NP showed a significant increase in newly synthesized proteoglycan in response to BMP-2. IVD cells from all zones demonstrated no significant expression of bone sialoprotein, DLX5, osteocalcin mRNA after treatment with BMP-2. Conclusion.: BMP-2 clearly exerted a mitogenic effect on AF cells, and stimulated proteoglycan synthesis in NP cells. However, BMP-2 did not have an osteogenic effect in any IVD region. Taken together, these results confirm that BMP-2 can be used as an anabolic agent for mitogenesis in AF cells and NP cell matrix regeneration without the possibility of osteogenesis.

AB - Study Design.: In vitro experiment using bone morphogenetic protein-2 (BMP-2) and cells from the nucleus pulposus (NP), transitional zone (TZ), and anulus fibrosus (AF) of the human intervertebral disc (IVD). Objective.: To demonstrate the differential effect of BMP-2 on DNA synthesis, proteoglycan synthesis, and osteocalcin mRNA expression in human IVD cells from the NP, TZ, and AF, respectively. Summary of Background Data.: BMP-2 has been proven to be effective in stimulating proteoglycan synthesis in articular chondrocytes and IVD cells from the NP. Nevertheless, the effect of BMP-2 on cells from different regions of the IVD has not yet been thoroughly elucidated. Methods.: Human IVDs were harvested from surgical disc procedures and tissue from the NP, TZ, and AF was obtained. Disc tissue was enzymatically digested, and IVD cells were cultured three-dimensionally in alginate beads. Then IVD cell cultures from the NP, TZ, and AF were exposed to BMP-2. DNA synthesis and newly synthesized proteoglycan were measured. Reverse transcription-polymerase chain reaction for mRNA expression of bone sialoprotein, DLX5, osteocalcin, and collagen type I, was performed. Results.: Cells from the AF responded to BMP-2 with mitogenesis. There was no significant increase in DNA synthesis in cultures from the NP and TZ treated with BMP-2. Only cells from the NP showed a significant increase in newly synthesized proteoglycan in response to BMP-2. IVD cells from all zones demonstrated no significant expression of bone sialoprotein, DLX5, osteocalcin mRNA after treatment with BMP-2. Conclusion.: BMP-2 clearly exerted a mitogenic effect on AF cells, and stimulated proteoglycan synthesis in NP cells. However, BMP-2 did not have an osteogenic effect in any IVD region. Taken together, these results confirm that BMP-2 can be used as an anabolic agent for mitogenesis in AF cells and NP cell matrix regeneration without the possibility of osteogenesis.

KW - Bone morphogenetic protein-2

KW - Intervertebral disc cell

KW - Proteoglycan synthesis

KW - Zonal effect

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